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Isotype pe conjugated igg1

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Isotype PE-conjugated IgG1 is a laboratory reagent used for flow cytometry applications. It serves as a control for non-specific antibody binding.

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2 protocols using isotype pe conjugated igg1

1

Long-term Immunophenotyping of hBM-MSCs

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hBM-MSC immunophenotypes were evaluated during long-term culture25 (link). Harvested cells were labeled using mouse anti-human monoclonal antibodies: phycoerythrin (PE)-conjugated CD105, CD90, CD73, CD34, CD45, CD11b and CD79a, and fluorescein isothiocyanate (FITC)-conjugated HLA-DR (BD Biosciences, San Jose, CA, USA). As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension containing 1 × 106 cells was incubated with monoclonal antibodies for 15 min at room temperature in the dark and fixed with BD Cytofix (BD Biosciences). The samples were analyzed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuest Proversion 6.0 software (BD Biosciences).
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2

Immunophenotyping of Mesenchymal Stem Cells

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Cells harvested from 3rd passage (P3) were used for immunophenotyping analysis. Mouse anti-human monoclonal antibodies: phycoerythrin (PE)-conjugated CD73, CD90, CD105, CD45, CD11b, CD34, CD79a, and FITC-conjugated HLA-DR (BD Biosciences, San Jose, CA) were used. As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension, containing 1 × 106 cells, were incubated with monoclonal antibodies at room temperature in the dark and fixed with BD CytofixTM (BD Biosciences). The samples were analyzed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuestTM Proversion 6.0 software (BD Biosciences).
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