The immunohistochemistry assay was performed according to Yin et al. (26 (link)). The sections were incubated with the primary antibody of CD68 (Ab955, 1: 100, Abcam, Cambridge, UK) at 4 °C overnight and horseradish peroxidase labeled goat anti-mouse IgG antibody (A205719, 1:200, Abcam, Cambridge, UK) at room temperature for 1 h. The color reaction was performed with diaminobenzidine chromogen solution (Dako, Carpinteria, USA). Brown-yellow particles represented the positive expression of CD68 protein and the blue particles represented the nucleus stained by hematoxylin (Sigma, USA).
Diaminobenzidine chromogen solution
Manufactured by Agilent Technologies
Sourced in United States
Diaminobenzidine chromogen solution is a laboratory reagent used for the detection and visualization of specific proteins or enzymes in various applications, such as immunohistochemistry and enzyme-linked immunosorbent assays (ELISA). The solution contains diaminobenzidine, a chromogenic substrate that undergoes a color reaction in the presence of the target analyte, producing a visible brown or reddish-brown stain. This solution is commonly used to facilitate the detection and analysis of target molecules in biological samples.
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Lab products found in correlation
3 protocols using diaminobenzidine chromogen solution
1
CD68 Immunohistochemistry Staining Protocol
2
Immunohistochemical Analysis of CD68 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The immunohistochemistry assay was performed according to Yin et al [26] . The sections were incubated with the primary antibody of CD68 (Ab955, 1: 100, Abcam, Cambridge, UK) at 4 °C overnight and horseradish peroxidase labeled goat anti-mouse IgG antibody (A205719, 1: 200, Abcam, Cambridge, UK) at room temperature for 1 h. The color reaction was performed with diaminobenzidine chromogen solution (Dako, Carpinteria, USA). Brown-yellow particles represented the positive expression of CD68 protein and the blue particles represented the nucleus stained by hematoxylin (Sigma, USA).
3
Immunohistochemical Analysis of CD68 Expression
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The immunohistochemistry assay was performed according to Yin et al [26] . The sections were incubated with the primary antibody of CD68 (Ab955, 1: 100, Abcam, Cambridge, UK) at 4 °C overnight and horseradish peroxidase labeled goat anti-mouse IgG antibody (A205719, 1: 200, Abcam, Cambridge, UK) at room temperature for 1 h. The Color reaction was performed with diaminobenzidine chromogen solution (Dako, Carpinteria, USA). Brown-yellow particles represented the positive expression of CD68 protein and blue particles represented the nucleus stained by hematoxylin (Sigma, USA).
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