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B16 f10 cancer cell line

The B16-F10 cell line is a murine melanoma cell line derived from C57BL/6 mice. It is a highly metastatic cell line commonly used in cancer research.

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2 protocols using b16 f10 cancer cell line

1

Syngeneic Mouse Tumor Cell Line Implantation

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For our tumor models, we used six different syngeneic mouse tumor cell lines. The CT26 cancer cell line was obtained from ATCC, maintained in RPMI-10% FBS at 37°C, and implanted into BALB/c mice subcutaneously at 1×106 cells per mouse. The MC-38 cancer cell line was obtained from NCI/NIH, maintained in RPMI-10% FBS at 37°C, and implanted into C57B6/J mice subcutaneously at 2×105 cells per mouse. The EMT6 cancer cell line was obtained from ATCC, maintained in RPMI-10% FBS at 37°C, and implanted into BALB/c mice subcutaneously at 2×105 cells per mouse. The LL2 cancer cell line was obtained from ATCC, maintained in RPMI-10% FBS at 37°C, and implanted into C57B6/J mice subcutaneously at 2×105 cells per mouse. The Sa1N cancer cell line was obtained from ATCC, maintained in Eagle’s Minimum Essential Medium (EMEM, ATCC 30–2003) at 37°C, and implanted into A/J mice subcutaneously at 1 ×106 cells per mouse. The B16-F10 cancer cell line as obtained from ATCC, maintained in RPMI-10% FBS at 37°C, and implanted into C57B6/J mice subcutaneously at 2×105 cells per mouse.
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2

Syngeneic Mouse Tumor Cell Line Implantation

Check if the same lab product or an alternative is used in the 5 most similar protocols
For our tumor models, we used six different syngeneic mouse tumor cell lines. The CT26 cancer cell line was obtained from ATCC, maintained in RPMI-10% FBS at 37°C, and implanted into BALB/c mice subcutaneously at 1×106 cells per mouse. The MC-38 cancer cell line was obtained from NCI/NIH, maintained in RPMI-10% FBS at 37°C, and implanted into C57B6/J mice subcutaneously at 2×105 cells per mouse. The EMT6 cancer cell line was obtained from ATCC, maintained in RPMI-10% FBS at 37°C, and implanted into BALB/c mice subcutaneously at 2×105 cells per mouse. The LL2 cancer cell line was obtained from ATCC, maintained in RPMI-10% FBS at 37°C, and implanted into C57B6/J mice subcutaneously at 2×105 cells per mouse. The Sa1N cancer cell line was obtained from ATCC, maintained in Eagle’s Minimum Essential Medium (EMEM, ATCC 30–2003) at 37°C, and implanted into A/J mice subcutaneously at 1 ×106 cells per mouse. The B16-F10 cancer cell line as obtained from ATCC, maintained in RPMI-10% FBS at 37°C, and implanted into C57B6/J mice subcutaneously at 2×105 cells per mouse.
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