The conjunctival swabs were transported and stored at room temperature. All the samples were tested immediately at the Institute of Medical Microbiology and Hygiene (University Hospital of Tübingen). Supplemented Columbia sheep blood agar plates (Oxoid GmbH, Wesel, Germany), Endo agar plates, and supplemented brain heart infusion (BHI) agar plates (Institute of Medical Microbiology and Hygiene) were incubated at 37°C to test for bacterial contamination. A liver broth was also used. Additionally, yeast-gentamicin plates (Institute of Medical Microbiology and Hygiene) were used to detect fungal contamination, and they were incubated at 30°C. The plates were incubated for 10 days. They were read after 24 and 48 hours and after 10 days.If there was cultural growth, the microorganisms were detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (Microflex; Bruker Corporation, Billerica, MA). Any proof of bacterial or fungal microorganisms was documented as contamination.
Endo agar plates
Manufactured by Thermo Fisher Scientific
Sourced in Germany
Endo agar plates are a microbiological culture medium used for the selective isolation and identification of Gram-negative bacteria, particularly Escherichia coli. They contain selective and differential agents that inhibit the growth of Gram-positive bacteria while allowing Gram-negative bacteria, such as E. coli, to grow and exhibit characteristic colonial morphologies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using endo agar plates
1
Evaluation of Conjunctival Swab Contamination
2
Examination of Conjunctival Swab Samples
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The conjunctival swabs were transported and stored at room temperature. All the samples were tested immediately at the Institute of Medical Microbiology and Hygiene (University Hospital of Tübingen). Supplemented Columbia sheep blood agar plates (Oxoid GmbH, Wesel, Germany), Endo agar plates, and supplemented brain heart infusion (BHI) agar plates (Institute of Medical Microbiology and Hygiene) were incubated at 37°C to test for bacterial contamination. A liver broth was also used. Additionally, yeast-gentamicin plates (Institute of Medical Microbiology and Hygiene) were used to detect fungal contamination, and they were incubated at 30°C. The plates were incubated for 10 days. They were read after 24 and 48 h and after 10 days.If there was cultural growth, the microorganisms were detected by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (Microflex; Bruker Corporation, Billerica, MA). Any proof of bacterial or fungal microorganisms was documented as contamination.
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