Rabbit anti β actin polyclonal antibody sc 130656

Aβ_25–35, a toxic fragment of the full-length Aβ peptide, was purchased from American Peptide Company (131602-53-4, United States), which needs to be solubilized in sterile water and aggregated at 37°C for 7 days before use. The siRNA transfection reagent was purchased from Guangzhou RiboBio Company Limited (C10511-1, Guangzhou, China). The Simple ChIP Enzymatic Chromatin IP Kit was obtained from Cell Signaling Technology (9003S, BOS, USA), and rabbit anti-acetylation of histone 3 lysine 9 (Ace-H3K9) polyclonal antibody for ChIP was from Merck Millipore (06942, Darmstadt, Germany). The rabbit anti-PPARγ polyclonal antibody (D262458), rabbit anti-HDAC2 polyclonal antibody (D155199), and rabbit anti-HDAC3 polyclonal antibody (D260481) were purchased from Bio Basic Inc. (Canada); rabbit anti-LPL polyclonal antibody (sc-32885) and rabbit anti-β-actin polyclonal antibody (sc-130656) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Goat anti-rabbit immunoglobulin G (lgG) secondary antibody was from Shanghai Sangon Biotech Company Limited (D111018, Shanghai, China). Cell culture medium was purchased from American Hyclone Inc. (SH30023.01B, USA).

β-hydroxybutyrate (βOHB) was purchased from Shanghai Yingxin Laboratory Equipment Co., Ltd. (Shanghai, China); rabbit anti-Aβ_1-42 polyclonal antibody (ab10148) was purchased from Abcam Inc. (Cambridge, United Kingdom); rabbit anti-HDAC3 polyclonal antibody (sc-11417) and rabbit anti-β-actin polyclonal antibody (sc-130656) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, United States). Rabbit anti-AQP4 polyclonal antibody (D160223), goat anti-rabbit immunoglobulin G (IgG) secondary antibody, and FITC-donkey anti-rabbit IgG secondary antibody were obtained from Shanghai Sangon Biotech Co., Ltd. (Shanghai, China). Cell culture medium was purchased from Hyclone Laboratories Inc. (United States). Aβ_25-35 (American Peptide Company, Inc., United States), a toxic fragment of the full-length Aβ peptide, was solubilized in sterile water and aggregated at 37°C for 7 days. Immunohistochemistry kits were obtained from Beijing Zhongshan Biotechnology (Beijing, China).