Trypsin v5113

Dithiothreitol (DTT, D9779), iodoacetamide (IAA), sodium dodecyl sulfate (SDS), trifluoroacetic acid (TFA), bovine ribonuclease B (R7884), bovine fetuin (F3004), bovine asialofetuin (A4781), and bovine α1-acid glycoprotein (G3643) were purchased from Millipore Sigma (Burlington, MA). Urea (U15), tris-base (BP152), hydrochloric acid (A144SI), formic acid (A117), water, acetonitrile, and PGC guard columns (35003–014001) were purchased from Fisher Scientific (Waltham, MA). Capillary tubing (1068150019) was purchased from PolyMicro. Trypsin (V5113) was purchased from Promega (Madison, WI). PolyHYDROXYETHYL-A packing material was purchased from PolyLC. Pencil column heater was purchased from Pheonix S&T. Sources of all other materials are noted.

Lysozyme from chicken egg white (SwissProt Accession #: P00698), bovine pancreatic ribonuclease (RNase A, SwissProt Accession #: P61823), BSA (SwissProt Accession #: P02769), N-ethylmaleimide (NEM), formic acid, and trifluoroacetic acid (TFA) were purchased from Sigma (St. Louis, MO). C₁₈ spin columns were purchased from Fisher Scientific (Fair Lawn, NJ). Trypsin (V5113) and pepsin (V195A) were purchased from Promega (Madison, WI). ACN, methanol, acetic acid, and water were purchased from J. T. Baker (Center Valley, PA). SDS, Tris-HCl, polyacrylamide, ammonium persulfate, TEMED, Laemmli buffer, and Coomassie Brilliant Blue were purchased from BioRad (Hercules, CA).