Cd163

Manufactured by ZSGB-BIO

Sourced in Japan

CD163 is a transmembrane glycoprotein that functions as a scavenger receptor. It is primarily expressed on the surface of tissue macrophages and monocytes. CD163 plays a role in the clearance of hemoglobin-haptoglobin complexes from the bloodstream.

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Lab products found in correlation

Cd206, by R&D Systems (2 mentions) Pg m1, by Agilent Technologies (2 mentions) Epr1344, by Abcam (2 mentions) Ep334, by ZSGB-BIO (2 mentions) Leum1, by ZSGB-BIO (2 mentions) Cd169, by R&D Systems (2 mentions) Vectra inform image analysis system, by Thermo Fisher Scientific (2 mentions) Cd11b, by Abcam (2 mentions) Envision system, by Agilent Technologies (2 mentions) Ctla4, by Abcam (1 mentions) Sp rabbit mouse hrp dab kit, by CWBIO (1 mentions) Pd l1, by Abcam (1 mentions)

4 protocols using cd163

Immunohistochemical Analysis of Immune Cells in HCC

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Paraffin-embedded and formalin-fixed HCC samples were cut into 5-μm sections, which were then processed for immunohistochemistry as previously described. Briefly, the sections were incubated with Abs against the following human antigens: CD8 (rabbit monoclonal, clone EP334, ZSBio, China), CD11b (rabbit monoclonal, clone EPR1344, Abcam, UK), CD68 (mouse monoclonal; clone PG-M1; Dako Cytomation, USA), CD15 (mouse monoclonal; clone LeuM1; ZSBio), CD206 (mouse monoclonal, clone 685645, R&D Systems, USA), CD204 (mouse monoclonal, clone SRA-C6, Transgenic, Japan), CD163 (mouse monoclonal, clone 10D6, ZSBio) and CD169 (sheep polyclonal, clone NS0, R&D Systems). Then, the sections were stained in an EnVision System (Dako Cytomation, USA). Positive cells were detected by microscopy and quantified using the Vectra-Inform image analysis system (Perkin-Elmer Applied Biosystems, Foster City, CA, USA).

Wang Y., Li Z., Huang Z., Yu X., Zheng L, & Xu J. (2022). C-Reactive Protein Is an Indicator of the Immunosuppressive Microenvironment Fostered by Myeloid Cells in Hepatocellular Carcinoma. Frontiers in Oncology, 11, 774823.

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Immunohistochemical Analysis of Immune Cells in HCC

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Muscle Biopsy Analysis Protocol

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During the operation, the surgeon performed paraspinal muscle biopsy of the multi dus muscle at the apex vertebra region. The biopsy tissue was wrapped in a semi-humid saline gauze and immediately transferred to the laboratory, where it was drained with blotting paper and was consecutively embedded in tragacanth and OCT compound (Tissue-Tek) and nally frozen in isopentane pre-cooled in liquid nitrogen. Cryostat sections were obtained with 7 to 10 µm thickening. Conventional H&E staining, histochemical staining (NADH-TR, Gomori trichrome, PAS, oil red O), and EnVision two-step immunohistochemical staining were performed under standard techniques that used the following primary antibodies: Dystrophin-1 (Anti-dystrophin rod domain, Leica Biosystems, USA), Dystrophin-2 (Anti-dystrophin C-terminal, Leica Biosystems, USA), Dystrophin-3 (Anti-dystrophin N-terminal, Leica Biosystems, USA), Myosin (Zsbio, China), MHC-1 (Zsbio, China), CD4 (Zsbio, China), CD8 (Zsbio, China), CD20 (Zsbio, China), and CD6 (Zsbio, China)8 or CD163 (Zsbio, China) antibodies respectively (10) .

Zheng D., Li J., Li J., Zhang Y., & Yu M. (2020). Special Pathologic Features of Adolescent Idiopathic Scoliosis: Could There be a New Type of Muscular Dystrophinopathy?.

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Immune Profiling of Pituitary Neoplasms

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Surgically resected PitNET specimens and NPs were xed in 4% paraformaldehyde for 24h and then embedded in para n. Para n blocks were cut into 4 µm sections and used for hematoxylin and eosin (HE) or immunohistochemistry (IHC) staining. Protein marker expression of immune cell in ltration, expression of immune checkpoint molecules, and PIT1 transcription factor were detected by SP Rabbit&Mouse HRP DAB Kit (catalog no. CW2069S, CWBIO). Slides were incubated with the following primary antibody: CD68 (catalog no.IR61361-2CN, DAKO), CD163 (catalog no.ZM-0428, ZSGB-BIO), CD4 (catalog no. RMA0620, MXBiotechnologies), CD8 (catalog no. ab4055, Abcam), PD-L1 (catalog no. ab205921, Abcam), PD-1 (catalog no. ZM-0381, ZSGB-BIO), CTLA4 (catalog no. ab237712, Abcam), PIT1 (catalog no.ZM0208, ZSGB-BIO), TPIT (catalog no.ZM-0316, ZSGB-BIO), SF1 (catalog no.ZM-0089, ZSGB-BIO). The estimation of PD-L1 expression followed the guideline of Consensus on the Immunohistochemical Tests in Solid Tumors (2021 version). Stained slides were digitally scanned using Slides Scanning Image System (TEKSQRAY). Images were analyzed using Image J software and quanti cation of positive expression was measured by two independent investigators (Mei Luo and Rui Tang). Cases with staining artifacts or poor tissue integrity following IHC processing were excluded.

Luo M., Tang R, & Wang H. (2023). Tumor immune microenvironment in pituitary neuroendocrine tumors (PitNETs): increased M2 macrophage infiltration and PD-L1 expression in PIT1-lineage subset. Journal of neuro-oncology, 163(3).

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