For migration and invasion assays, Matrigel-noncoated and Matrigel-coated chambers (BD Biosciences, USA) containing 8 μm pores were used for the assays. Briefly, 5 × 104 (1 × 105 cells for invasion) cells were seeded into the Matrigel-noncoated upper chambers (coated in Matrigel for invasion) in serum-free medium. The lower chamber of the transwell was filled with culture media containing 10% FBS as a chemo-attractant. After the chambers were incubated at 37 °C for 20 h, non-migrated (or non-invaded) cells on the top of the transwell were scraped off with a cotton swab. And then cells successfully translocated were fixed with 4% paraformaldehyde, stained with 0.1% crystal violet, and counted under a light microscope.
Matrigel noncoated
Manufactured by BD
Sourced in United States
Matrigel-noncoated is a laboratory product manufactured by BD. It is a soluble basement membrane extract purified from Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. The product provides an extracellular matrix that supports the attachment and differentiation of various cell types in cell culture applications.
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Lab products found in correlation
2 protocols using matrigel noncoated
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Cell Migration and Invasion Assay
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Cell Migration and Invasion Assay
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To evaluate the cell migration and invasive capacity of cells, Transwell assays were performed using Matrigel noncoated and Matrigel-coated Boyden chamber (BD Biosciences), respectively. Stable cells were serum-starved overnight, harvested, and resuspended in the serum-free medium. Cells (1×105) were then plated to the upper chamber. The medium containing 10% FBS was added to the lower chamber. After incubation at 37°C for 24 hours, the migrant and invasion cells attached to the lower surface of the membrane were fixed with formalin, stained with 0.5% crystal violet, and counted using a microscope. The experiments were performed in triplicate.
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