Cell lysates were prepared using RIPA lysis butter (P0013B, Beyotime), and the protein concentration was calculated with a BCA Protein Assay Kit (P0012, Beyotime). Protein samples were separated by SDS-PAGE electrophoresis and then electro-transferred onto PVDF membranes (1620184, Bio-Rad, Hercules, California). Five percent skim milk was used to block the membranes in TBST for 2 hours before incubating with primary antibodies including Cyclin B1 (1:1000, 12231S, CST, Danvers, Massachusetts), Cyclin D1 (1:1000, 55506S, CST), Cyclin Dependent Kinase 1 (CDK1, 1:1000, 77055S, CST), YAP (1:1000, 14074S, CST), p-YAP (1:1000, 13008S, CST), TAZ (1:1000, 83669S, CST), TEAD1 (1:500, sc-376113, Santa Cruz, Santa Cruz, California), Aggrecan (1:1000, 13880-1-AP, Proteintech, Chicago, Illinois), Collagen Type II (1:1000, 28459-1-AP, Proteintech), Connective Tissue Growth Factor (CTGF, 1:500, sc-365970, Santa Cruz) and Matrix Metallopeptidase 13 (MMP13, 1:1000, 18165-1-AP, Proteintech) at 4°C overnight. Membranes were incubated with secondary antibodies (1:1000, SA00001-2, Proteintech) at room temperature for 1 hour. After three washes with TBST, immunolabeling was activated by an Omni-ECL™ Femto Light Chemiluminescence Kit (SQ201, Epizyme, Shanghai, China) and captured by a GeneGnome XRQ analyzer (Syngen, Cambridge, UK). Gray value was analyzed using ImageJ normalized to GAPDH.
Sc 376113
Manufactured by Santa Cruz Biotechnology
Sc-376113 is a laboratory instrument for performing specialized scientific analyses. It is designed to aid in the study and understanding of biological processes and materials. The core function of this product is to provide accurate and reliable data to support research and development efforts. No further details regarding its intended use or interpretation of its capabilities are provided.
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Lab products found in correlation
2 protocols using sc 376113
1
Western Blot Analysis of Cell Signaling
2
Western Blot Analysis of Cell Signaling
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Cell lysates were prepared using RIPA lysis butter (P0013B, Beyotime), and the protein concentration was calculated with a BCA Protein Assay Kit (P0012, Beyotime). Protein samples were separated by SDS-PAGE electrophoresis and then electro-transferred onto PVDF membranes (1620184, Bio-Rad, Hercules, California). Five percent skim milk was used to block the membranes in TBST for 2 hours before incubating with primary antibodies including Cyclin B1 (1:1000, 12231S, CST, Danvers, Massachusetts), Cyclin D1 (1:1000, 55506S, CST), Cyclin Dependent Kinase 1 (CDK1, 1:1000, 77055S, CST), YAP (1:1000, 14074S, CST), p-YAP (1:1000, 13008S, CST), TAZ (1:1000, 83669S, CST), TEAD1 (1:500, sc-376113, Santa Cruz, Santa Cruz, California), Aggrecan (1:1000, 13880-1-AP, Proteintech, Chicago, Illinois), Collagen Type II (1:1000, 28459-1-AP, Proteintech), Connective Tissue Growth Factor (CTGF, 1:500, sc-365970, Santa Cruz) and Matrix Metallopeptidase 13 (MMP13, 1:1000, 18165-1-AP, Proteintech) at 4°C overnight. Membranes were incubated with secondary antibodies (1:1000, SA00001-2, Proteintech) at room temperature for 1 hour. After three washes with TBST, immunolabeling was activated by an Omni-ECL™ Femto Light Chemiluminescence Kit (SQ201, Epizyme, Shanghai, China) and captured by a GeneGnome XRQ analyzer (Syngen, Cambridge, UK). Gray value was analyzed using ImageJ normalized to GAPDH.
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