Cosmic calf serum (CCS) was
obtained from Hyclone Laboratories (South Logan, UT). Gentamicin was
from Lonza (Wakersville, MD). The 100× antibiotic–antimycotic
(Ab/Am) and TrypLE were from Life Technologies (Gaithersburg, MD).
Eagle’s Minimum Essential Medium (EMEM) and Leibovitz’s
L-15 medium were purchased from the American Type Culture Collection
(ATCC) (Manassas, VA). Matrigel and mineral oil were obtained from
VWR International (Radnor, PA). Polydimethylsiloxane (PDMS) prepolymer
(Sylgard 184) was from Dow Corning (Midland, MI). All solutions were
prepared using ultrapure DI water (NANOpure Diamond System, Barnstead
International, Dubuque, IA) and filtered using 0.2 μm nylon
syringe filters (Pall Corporation, Port Washington, NY). All other
reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless
stated otherwise.
obtained from Hyclone Laboratories (South Logan, UT). Gentamicin was
from Lonza (Wakersville, MD). The 100× antibiotic–antimycotic
(Ab/Am) and TrypLE were from Life Technologies (Gaithersburg, MD).
Eagle’s Minimum Essential Medium (EMEM) and Leibovitz’s
L-15 medium were purchased from the American Type Culture Collection
(ATCC) (Manassas, VA). Matrigel and mineral oil were obtained from
VWR International (Radnor, PA). Polydimethylsiloxane (PDMS) prepolymer
(Sylgard 184) was from Dow Corning (Midland, MI). All solutions were
prepared using ultrapure DI water (NANOpure Diamond System, Barnstead
International, Dubuque, IA) and filtered using 0.2 μm nylon
syringe filters (Pall Corporation, Port Washington, NY). All other
reagents were purchased from Sigma-Aldrich (St. Louis, MO) unless
stated otherwise.