EpCAM × CD3 BiTE concentrations were quantified by c-tag ELISA developed in house. Briefly, 96-well streptavidin-coated plates (Thermo Fisher) were incubated with biotinylated anti-c-tag conjugates (100 μl/well at 10 μg/ml; Thermo Fisher) diluted in PBS overnight at 4 °C. After blocking the plates with 20% newborn calf serum in PBS, serum samples and protein standards were added to the wells for incubation. Recombinant mouse EpCAM Fc chimera (Bio-Techne) was added to the wells at 5 μg/ml. After extensive washing, HRP Rat anti-Mouse IgG2a antibody (1:7500; Thermo Fisher) was added to the wells. After incubation with TMB substrates, the reaction was stopped, and ODs were determined at 450 nm with wavelength correction at 630 nm using a Synergy H4 Microplate Reader (BioTek). Samples were assayed in duplicate. For in vitro studies, samples were normalized to average values obtained from wells containing cells transfected by control nanoparticles.
96 well streptavidin coated plates
Manufactured by Thermo Fisher Scientific
The 96-well streptavidin-coated plates are a versatile laboratory tool designed for a range of bioassay applications. The plates feature a uniform coating of streptavidin, a high-affinity protein that binds to biotinylated molecules. This allows for the capture and immobilization of biotinylated targets, such as proteins, peptides, or nucleic acids, on the plate surface.
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Lab products found in correlation
2 protocols using 96 well streptavidin coated plates
1
Quantification of EpCAM x CD3 BiTE by c-tag ELISA
2
Quantification of EpCAM x CD3 BiTE by c-tag ELISA
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