Amber glass jars

On the second day of sequential sampling, pre-shift and post-shift hand wipe samples were collected from worker’s hands. Two 3”× 3” sterile gauze pads (Dynarex, Orangeburg, NY) were placed in 120 mL amber glass jars (Fisher Scientific, Pittsburgh, PA). Each jar included 6 mL of 99% HPLC grade isopropanol (Fisher Scientific) using an automatic pipette. The jars were then tightly sealed, and stored at approximately 5°C for up to seven days. Samples were collected in a break room or conference room before and after the work shift. During sample collection participants were instructed to grab one of the gauze pads and wipe both sides of their bare hands (the area from the bend of the wrist to the fingertips) for 30 seconds. Then they were instructed to grab the other wipe and repeat the process. Both gauze pads were placed into the same jar, sealed, and stored at refrigerated temperatures until analyzed. At the end of the day, workers were asked how many times they washed their hands that work day and glove use was observed and recorded.

Workers provided pre-shift and post-shift hand wipe samples on the second day of sampling. Sample jars were prepared less than a week before sampling. Two 3”x 3” sterile gauze pads (Dynarex, Orangeburg, NY) were placed in 120 mL amber glass jars (Fisher Scientific, Pittsburgh, PA). In each jar, we pipetted 6 mL of 99% HPLC grade isopropanol (Fisher Scientific). The jars were then tightly sealed and stored at approximately 5°C. Hand wipe samples were collected in a break room before and after the work shift. For sample collection, an industrial hygienist instructed participants to remove gloves, grab one of the gauze pads and wipe both bare hands for 30 seconds. Then they were instructed to grab the other wipe and repeat the process. Both gauze pads were placed back into the jar, sealed, and refrigerated (< 4C) until analyzed. For the post shift hand wipes, workers were asked if they washed their hands since providing the pre shift hand wipe sample. The hand wipe samples were analyzed for TPhP.