YSK05 (1-methyl-4,4-bis(((9Z,12Z)-octadeca-9,12-dien- 1-yl)oxy)piperidine) and YSK12-C4 (6Z,9Z,28Z,31Z)–19-(4-(dimethyl-amino)butyl)heptatriaconta-6,9,28,31 tetraen-19-ol) lipids were synthesized in our laboratory as described previously.33 (link),36 (link) Cholesterol (Chol) was purchased from Sigma-Aldrich (St Louis, MO, USA). 1,2-Dimirystoyl-sn-3-glycero methoxypolyethyleneglycol 2000 ether (mPEG2k-DMG) was purchased from the NOF Corporation (Tokyo, Japan). RiboGreen, DiI, and DiD were purchased from Molecular Probes (Eugene, OR, USA). The TRIzol reagent was purchased from Invitrogen (Carlsbad, CA, USA). The 6-(p-Toluidino)-2-naphthalenesulfonic acid (TNS) was purchased from Wako Pure Chemical Industries Ltd (Osaka, Japan). The sequences for the sense and antisense strands of siRNAs used in this study are listed in Table S1 . All the siRNAs used in this study were obtained from Hokkaido System Science (Sapporo, Japan). The sequences for the forward and reverse primers used in this study are listed in Table S2 . All primers were obtained from SIGMA Genosys Japan (Ishikari, Japan).
6 p toluidino 2 naphthalenesulfonic acid tns
Manufactured by Fujifilm
Sourced in Japan, United States
6-(p-Toluidino)-2-naphthalenesulfonic acid (TNS) is a chemical compound used as a fluorescent probe in various laboratory applications. It is a naphthalene derivative with a toluidine substituent and a sulfonic acid group. TNS exhibits changes in its fluorescence properties upon binding to proteins or other biological macromolecules, making it a useful tool for studying molecular interactions and dynamics in research settings.
Automatically generated - may contain errors
Lab products found in correlation
Ribogreen, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Cholesterol chol, by Merck Group (1 mentions)
Fitc conjugated isolectin b4, by Vector Laboratories (1 mentions)
Dual luciferase reporter assay reagent, by Promega (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanine perchlorate dii, by Thermo Fisher Scientific (1 mentions)
1 1 dioctadecyl 3 3 3 3 tetramethylindodicarbocyanine perchlorate did, by Thermo Fisher Scientific (1 mentions)
Hct116 human colorectal carcinoma cells, by American Type Culture Collection (1 mentions)
2 protocols using 6 p toluidino 2 naphthalenesulfonic acid tns
1
Lipid Synthesis and Reagent Procurement
2
Fluorescent Probes for Cell Imaging
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EverFluor TMR-X was purchased from Setareh Biotech (Eugene, OR, USA).
6-(p-Toluidino)-2-naphthalenesulfonic acid (TNS) was obtained from Wako Chemicals (Osaka, Japan). Ribogreen, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate (DiD) were obtained from Molecular Probes (Eugene, OR, USA). TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, USA). Dual-Luciferase Reporter Assay Reagent was purchased from Promega Corporation (Madison, WI, USA). FITC-conjugated Isolectin B4 was purchased from Vector Laboratories (Burlingame, CA). HeLa human cervical carcinoma cells was purchased from RIKEN Cell Bank (Tsukuba, Japan). HCT116 human colorectal carcinoma cells was purchased from ATCC (Manassas, VA, USA). All siRNAs ware obtained from Hokkaido System Science Co. Ltd. (Sapporo, Japan). The sequences for the sense and antisense strands of the siRNAs used in this study are listed in Supplementary Table.
6-(p-Toluidino)-2-naphthalenesulfonic acid (TNS) was obtained from Wako Chemicals (Osaka, Japan). Ribogreen, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) and 1,1'-dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine perchlorate (DiD) were obtained from Molecular Probes (Eugene, OR, USA). TRIzol reagent was obtained from Invitrogen (Carlsbad, CA, USA). Dual-Luciferase Reporter Assay Reagent was purchased from Promega Corporation (Madison, WI, USA). FITC-conjugated Isolectin B4 was purchased from Vector Laboratories (Burlingame, CA). HeLa human cervical carcinoma cells was purchased from RIKEN Cell Bank (Tsukuba, Japan). HCT116 human colorectal carcinoma cells was purchased from ATCC (Manassas, VA, USA). All siRNAs ware obtained from Hokkaido System Science Co. Ltd. (Sapporo, Japan). The sequences for the sense and antisense strands of the siRNAs used in this study are listed in Supplementary Table.
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