Arpe 19

Manufactured by BNCC

Sourced in China

ARPE-19 is a human retinal pigment epithelial cell line. It is commonly used in research related to the retinal pigment epithelium and associated eye conditions.

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Lab products found in correlation

Phosphate buffered saline (pbs), by Sangon (1 mentions) Fetal bovine serum (fbs), by Sangon (1 mentions) Trypsin, by Sangon (1 mentions) Dmem f12, by Procell (1 mentions) Arpe 19, by Procell (1 mentions) Antibiotic antimycotic, by Sangon (1 mentions) D glucose, by Sangon (1 mentions)

2 protocols using arpe 19

ARPE-19 Cells: H2O2-Induced Apoptosis

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Human retinal pigment epithelial cells (ARPE-19) were purchased as frozen vials from BeNa Culture Collection (Beijing, China), and all cell experiments were performed between the third and fifth generations. The cells were cultured in DMEM/F-12 (supplemented with 10% FBS, 1% streptomycin/penicillin) at 37 °C in a humidified atmosphere containing with 95% air and 5% CO₂. Cells at 80–90% confluence were selected for subculture and subsequent experimentation. For H₂O₂-induced apoptotic studies, the cells were cultured overnight in DMEM/F12 containing 2% FBS and then incubated in serum-free medium for 30 min, followed by exposure to H₂O₂ (400 μM) for 6 h. Catalpol was dissolved in DMSO and stored at 4 °C. Under the experimental conditions, the concentration of DMSO was maintained below 0.1%, since our previous studies revealed that this concentration does not cause cellular damage.

You L., Peng H., Liu J., Cai M., Wu H., Zhang Z., Bai J., Yao Y., Dong X., Yin X, & Ni J. (2021). Catalpol Protects ARPE-19 Cells against Oxidative Stress via Activation of the Keap1/Nrf2/ARE Pathway. Cells, 10(10), 2635.

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High-glucose-induced ARPE-19 cell model

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We used the Human retinal pigment epithelial (ARPE-19) cell line bought from BeNa Culture Collection (Beijing, China). The ARPE-19 cells were cultured using DMEM/F12 (Procell, China) medium with 10% fetal bovine serum (Sangon Biotech, Shanghai, China) and 1% Antibiotic-Antimycotic (Sangon Biotech) at 37°C with 5% CO 2 . When the cell density maintained 80% to 90%, we washed the cells with PBS (Sangon Biotech) and passaged them with 0.05% trypsin (1:3) (Sangon Biotech). Then, the cells were cultured in the medium containing 30 mmol/L D-glucose (Sangon Biotech) to establish a high-glucose model. After culturing for 48 hours, the cell samples were collected for subsequent experiments. Kaempferol was diluted into series of concentrations (5, 10, 15, 20, 25 μM) with the basal medium.

Lin X., Bao M., Zhang X., Qirula S., Jiao C., Zhang D, & Han J. (2024). Study on the bioactive ingredients and mechanism of Huangqi against diabetic retinopathy based on network pharmacology and experimental verification. Journal of the Chinese Medical Association : JCMA, 87(8).

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