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Rabbit anti synaptophysin 101 002 and rabbit anti vgat 131 002

Manufactured by Synaptic Systems

Rabbit anti-synaptophysin (101 002) is an antibody that recognizes the synaptophysin protein, a marker for synaptic vesicles. Rabbit anti-vGAT (131 002) is an antibody that recognizes the vesicular GABA transporter, a marker for GABAergic presynaptic terminals.

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2 protocols using rabbit anti synaptophysin 101 002 and rabbit anti vgat 131 002

1

Generation of Molecular Reagents for Cell Biology

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The sources of cDNAs were as follows: pEGFPC-Sac2 and pSNAP-human CLC were generated in our laboratory, and pFUGW-GFP-Sac2 was generated from pEGFPC-Sac2 by Janelia Research Campus/HHMI. The following primary antibodies used in this study were generated in our laboratory: rabbit anti-SJ1 and rabbit anti-auxilin. Antibodies obtained from commercial sources were as follows: mouse anti-EEA1 from BD Biosciences; mouse anti-amphiphysin 2, rabbit anti-CLC, guinea pig anti-vGluT1 (AB5905), rabbit anti-TH (AB152), and rat anti-DAT (MAB369) from Millipore Sigma; rabbit anti-synaptophysin (101 002) and rabbit anti-vGAT (131 002) from Synaptic Systems; rat anti-LAMP1 (ID4) from Developmental Studies Hybridoma Bank; rabbit anti-BAG3 (10599-1-AP) from Proteintech, mouse anti-tubulin (T5168) from Millipore Sigma; rabbit anti-tubulin (PA1-21153) from Invitrogen; mouse anti-parkin (4211) from Cell Signaling Technology; and Alexa Fluor 488-, 594- and 647-conjugated secondary antibodies from Invitrogen. The rabbit anti-Sac2 antibody was a kind gift from Yuxin Mao, Cornell University.
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2

Comprehensive Antibody Characterization for Neurobiology

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The sources of cDNAs were as follows: pEGFPC-Sac2 and pSNAP-human clathrin light chain (CLC) were generated in our lab; pFUGW-GFP-Sac2 was generated from pEGFPC-Sac2 by Janelia Research Campus/HHMI. The following primary antibodies used in this study were generated in our lab: rabbit anti-SJ1, rabbit antiauxilin. Antibodies obtained from commercial sources were as follows: mouse anti-EEA1 from BD biosciences; mouse anti-amphiphysin 2, rabbit anti-CLC, guinea pig anti-vGluT1 (AB5905), rabbit anti-TH (AB152), and rat anti-DAT (MAB369) from Millipore; rabbit anti-synaptophysin (101 002) and rabbit anti-vGAT (131 002) from Synaptic Systems; rat anti-LAMP1 (ID4) from DSHB; rabbit anti-BAG3 (10599-1-AP) from Proteintech, mouse anti-tubulin (T5168) from Sigma; rabbit anti-tubulin (PA1-21153) from Invitrogen; mouse anti-parkin (4211) from Cell Signaling Technology; Alexa/488-, 594-and 647-conjugated secondary antibodies from Invitrogen. The rabbit anti-Sac2 antibody was a kind gift of Yuxin Mao, (Cornell University, NY).
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