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Gelatin type b

Manufactured by Thermo Fisher Scientific
Sourced in United States

Gelatin type B is a purified protein substance derived from the partial hydrolysis of collagen extracted from animal skin and bones. It is commonly used as a gelling agent in various laboratory applications.

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2 protocols using gelatin type b

1

VEGFR2 Signaling Pathway Analysis

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Antibodies against phospho-tyrosine (PY99), Gab1 (Clone H-198), and VEGFR2 (C-1158) were purchased from Santa Cruz Biotechnology. Anti-pS473 Akt, Akt, pY783 PLCγ, PLCγ, pT202/Y204ERK1/2, ERK1/2, pY1175 VEGFR2, pY951 VEGFR2, Horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgGs were purchased from Cell Signalling. Antibodies against Rac1 and Matrigel were purchased from BD Biosciences. CdGAP antibody (HPA036380) was purchased from Sigma. VEGFR2 mouse antibody used for western blotting was purchased from Upstate (Clone CH-11). Gelatin type B was from Fisher Scientific. Recombinant human VEGF-A was obtained from the Biological Resources Branch Preclinical Repository of the National Cancer Institute - Frederick Cancer Research and Development Center. Transwell filters (poly-carbonate membrane, 8 μm pore-size) were from Corning.
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2

Synthesis and Characterization of LMN-NKA

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Gelatin type B and glycine (ACS agent, 98.5%) were purchased from Fisher Scientific (Pittsburg, PA, USA). Sorbitol Solution USP (70%) was obtained from Spectrum Chemical MFG Corp (Gardena, CA, USA). HPLC grade trifluoroacetic acid and acetonitrile were purchased from Sigma-Aldrich (St. Louis, MO, USA). LMN-NKA was synthesized for Dignify Therapeutics (Durham, NC, USA) by Bachem America Inc (Torrance, CA, USA). GR159897 (NK2 receptor antagonist; Tocris, Bristol, United Kingdom) was solubilized at 50 mM in DMSO and diluted with saline to a concentration of 1 mg/mL.
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