Salts, solvents, and HEPES were purchased from Sigma-Aldrich. R(+)-Etomidate was purchased from Toronto Research Chemicals Inc (North York, Ontario, Canada). GABA, propofol, phenobarbital, DMEM high glucose, fetal bovine serum, and 0.01% poly-L-Lys solution (70-150 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The steroid pregnanolone (3α5βP) was purchased from BioTechne Corp. (Minneapolis, MN, USA). Lipofectamine 2000, Opti-MEM, and trypsin-EDTA were obtained from Thermo Fischer Scientific (Waltham, MA, USA). NS-1738 and PNU-120596 were obtained from Cayman Chemical (Ann Arbor, MI, USA) and Adooq Bioscience (Irvine, CA, USA). PAM-2 was synthesized as described previously (Arias et al., 2011 (link)). The structures of NS-1738, PAM-2, and PNU-120596 are provided in Fig. 1 . Key protein targets and ligands in this article are hyperlinked to corresponding entries in http://www.guidetopharmacology.org , and are permanently archived in the Concise Guide to PHARMACOLOGY 2021/22 (Alexander et al., 2021 (link)).
Poly l lys solution 70 150 kda
Manufactured by Merck Group
Sourced in United States
0.01% poly-L-Lys solution (70-150 kDa) is a laboratory reagent. It is an aqueous solution containing 0.01% by weight of poly-L-lysine with an average molecular weight range of 70-150 kilodaltons.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Dmem high glucose, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Opti mem, by Thermo Fisher Scientific (2 mentions)
Hepes, by Merck Group (2 mentions)
Propofol, by Merck Group (2 mentions)
Phenobarbital, by Merck Group (1 mentions)
Ns 1738, by Cayman Chemical (1 mentions)
Tetrahydroquinoline, by Merck Group (1 mentions)
Tetrahydroisoquinoline, by Merck Group (1 mentions)
Triethylamine, by Fluorochem (1 mentions)
N 3 dimethylaminopropyl n ethylcarbodiimide hydrochloride edc, by Fluorochem (1 mentions)
2 protocols using poly l lys solution 70 150 kda
1
Neurosteroid and Allosteric Modulator Testing
2
Synthesis and Characterization of Gabazine-Oregon Green
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Drugs R(+)-Etomidate (etomidate) was purchased from Toronto Research Chemicals Inc. (North York, Ontario, Canada) . The salts used in buffers, HEPES, GABA, propofol, DMEM high glucose, fetal bovine serum, and 0.01% poly-L-Lys solution (70-150 kDa) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Pregnenolone sulfate was purchased from Bio-Techne Corp. (Minneapolis, MN, USA) and Sigma-Aldrich. Phenobarbital was obtained from J.T. Baker Chemical Co (Phillipsburg, NJ, USA). Lipofectamine 2000, Opti-MEM, and trypsin-EDTA were obtained from Thermo Fischer Scientific (Waltham, MA, USA). (E)-3-(furan-2-yl)acrylic acid, (E)-3-(2thienyl)acrylic acid, 1-hydroxybenzotriazole hydrate (HOBt), p-toluidine, tetrahydroquinoline, tetrahydroisoquinoline and 1-aminoindoline hydrochloride were obtained from Sigma-Aldrich SRL (Milan, Italy) .
N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), triethylamine, sodium hydride and iodomethane were purchased from Fluorochem (Glossop, UK).
Gabazine-oregon green (Gzn-OG) was synthesized as described previously (Sakamoto et al., 2019) .
N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), triethylamine, sodium hydride and iodomethane were purchased from Fluorochem (Glossop, UK).
Gabazine-oregon green (Gzn-OG) was synthesized as described previously (Sakamoto et al., 2019) .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!