Sperm samples were prepared using density gradient technique according to manufacturer’s protocol (Sperm Gradient kit, COOK medical, Australia). Briefly, density gradient media was prepared by layering the 40% medium over the 80% medium, and two ml of the liquefied semen sample were added over the upper layer. After centrifugation (380 g for 10 minutes), most of the supernatant was gently removed and the pellet was resuspended in two ml of Sperm Medium (Sperm Medium, COOK medical, Australia) to remove the density gradient medium. At the end of the centrifugation (670 g for 5 minutes), the final pellet was resuspended in the Sperm Medium.
Sperm medium
Manufactured by Cook Medical
Sourced in Australia
Sperm Medium is a lab equipment product designed to support the survival and motility of sperm cells during assisted reproductive procedures. It provides a balanced, isotonic solution to maintain the optimal environment for sperm during handling and processing.
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Lab products found in correlation
2 protocols using sperm medium
1
Sperm Preparation Using Density Gradient
2
Sperm Motility Enrichment via Swim-up
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From each semen sample, one aliquot was used to prepare the motile sperm—enriched fraction using the swim-up technique [49 (link)]. Briefly, one volume of semen was placed in a tube and was overlaid by one volume of culture medium (Sperm Medium, COOK medical, Australia) according to the manufacturer’s instructions. The tube was incubated for 45 min at 37°C under 5% CO2, 5% O2 and 90% N2. The supernatant was aspirated and transferred to an empty tube: replicate measurements of sperm concentration, sperm motility, and sperm morphology were performed according to the World Health Organization criteria 2010 [48 ] (Fig 1 ).
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