Asc 67824s

Tumor samples were fixed with 4% paraformaldehyde at room temperature for 24 h. Then, paraffin sections (5 μm) were collected for the IHC assay. Sections were stained by NLRP3 (ab214185, Abcam, Cambridge, UK), ASC (67824s, Cell Signaling Technology, Inc., Danvers, MA, USA), IL-1β (2242s, CST), IL-18 (ab71495, Abcam), MDA (LS-Bio 188950, LS-Bio, Shanghai China), pro-casepase-1 (ab179515, Abcam), PKC-α (ab32376, Abcam) and BCL2L1 (A00181, Boster, Wuhan, China) antibody, respectively.

Gentiopicroside (Molecular formula: C₁₆H₂₀O₉, Molecular weight: 356.32 g·mol⁻¹, batch no: GR-135-180320, purity ≥ 98%) was from Nanjing Guangrun Biotechnology Co., Ltd. (Nanjing, China). Dexamethasone tablets (Dex, batch no: 160601201) were from Chen Xin Pharmaceutical Co., Ltd. (Jining, China). Dexamethasone powder (D4902, purity ≥ 97%) was from Sigma-Aldrich (MO, USA). Complete Freund’s adjuvant (CFA, 7027) was from Chondrex, Inc. (MA, USA). Recombinant human TNF-α (P00029), and IL-6 (SEKH-0013) and IL-1β (SEKH-0002) ELISA kits were from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). The Cell Light™ EdU Apollo^®488 In Vitro Imaging Kit (C10310-3) was from RiboBio (Guangzhou, China). The Reactive Oxygen Species Kit (S0033) was from Beyotime Institute of Biotechnology (Haimen, China). Antibodies specific for IκBα (L35A5), p-IκBα (2859S), NF-κB p65 (8242S), p-p65 (3033S), Vimentin (5741T), NLRP3 (15101S), and ASC (67824S) were from Cell Signaling Technology (MA, USA). Anti-CD68 (NB100-683SS) was from Novus Biologicals (CO, USA). Anti-caspase-1 (NBP1-76605) was from RD system (MN, USA). All other chemicals were analytical grade.