Cangrelor ar c69931mx

Adenosine receptor agonists were purchased from Sigma (St. Louis, MO, USA) (NECA (CAS № 35920-39-9)), and Cayman (Ann Arbor, MI, USA) (regadenoson (CAS № 313348-27-5)). LUF5835 (2-amino-6-(1H-imidazol-2-ylmethylsulfanyl)-4-(3-hydroxy-phenyl) pyridine-3,5dicarbonitrile) was synthesized at Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland. Cangrelor (AR-C69931MX) was from Cayman Chemical (Ann Arbor, MI, USA). Prasugrel metabolite (R-138727) was obtained from BOC Sciences (Shirley, NY, USA). Calcein AM was obtained from Molecular Probes (Eugene, OR, USA). Antibodies anti-human CD61/PErCP, CD61/PE, CD62/PE, PAC-1/FITC, mouse IgG1/PE isotype control, mouse IgG1/FITC isotype control, Cellfix, buffered sodium citrate was purchased from Becton-Dickinson (San Diego, CA, USA). Phosphate buffered saline pH 7.4 (PBS) was obtained from Corning (New York, NY, USA). Dimethyl sulfoxide (DMSO), adenosine diphosphate (ADP), and bovine serum albumin (BSA) were obtained from Sigma (St. Louis, MO, USA). All other chemicals, unless otherwise stated, were purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland).

The adenosine receptor agonist—HE-NECA (CAS № 141018-30-6) was purchased form Abcam (Cambridge, United Kingdom), and cangrelor (AR-C69931MX) from Cayman Chemical (Ann Arbor, MI, USA). Prasugrel and prasugrel metabolite (R-138727) were purchased from BOC Sciences (Shirley, NY, USA). Dimethyl sulfoxide (DMSO) and adenosine diphosphate (ADP) were obtained from Sigma (St. Louis, MO, USA). Ferric chloride (FeCl₃) was obtained from POCh (Gliwice, Poland). Oregon Green 488-labelled human fibrinogen was from Molecular Probes (Eugene, OR, USA). Type I collagen from equine tendon was purchased from Chrono-log Corp. (Havertown, PA, USA) D-phenylalanyl-prolyl-arginyl chloromethyl ketone (PPACK), was from Calbiochem (Darmstadt, Germany). Anti-CD41 phycoerythrin (PE)-conjugated antibodies were purchased from Becton-Dickinson (San Diego, CA, USA).
A 100 mM stock solution of HE-NECA was prepared in DMSO. During platelet aggregometry measurements, aliquots of the stock solution were then diluted with PBS to working concentrations not exhibiting precipitates, as noted by Boncler et al. [9 (link)], and added to the biological material. The dilution factor was chosen in such a way to yield a maximal concentration of DMSO never exceeding 0.1% in a biological sample in any of the assays.