Earle s salts and non essential amino acids

Human K562 cells were cultured in RPMI-1640 media at 37 °C, supplemented with 10% heat-inactivated FBS (Biosera, UK), 1% l-glutamine and 1% penicillin–streptomycin. Cells were washed in low conductivity DEP buffer medium containing 8.5% sucrose and 0.3% dextrose and resuspended in CPM at a final concentration of 10⁶ cells/ml. HeLa cells were cultured in MEM with Earle’s salts and non-essential amino acids (Biosera, UK) supplemented with 10% heat-inactivated FBS (Sigma-Aldrich, UK), 1% l-glutamine and 1% penicillin–streptomycin (Sigma Aldrich, UK). At about 80% confluency, cells were trypsinized and washed twice in DEP buffer medium and resuspended in CPM at a final concentration of 10⁶ cells/ml. HL-1 cells were cultured in Claycomb media supplemented with 10% heat-inactivated FBS, 1% penicillin–streptomycin, 1% l-glutamine and Norepinephrine (0.1 mM) (Biosera, UK). The cells were trypsinised at 100% confluence and washed twice in DEP buffer medium before resuspending in CPM at a final concentration of 10⁶ cells/ml. Viability of cell suspensions was confirmed on control samples prior to experiment using a LIVE/DEAD Staining Kit (Sigma Aldrich, UK). Further viability was monitored with trypan blue (Sigma Aldrich, UK).