For the preparation of lipid nanoparticles, the following materials were used: chitosan low molecular weight (LCS; MW = 50,000–190,000 Da; 20–300 cP, 1% w/w in 1% acetic acid, 25 °C, Brookfield; 75–85% deacetylated), chitosan medium molecular weight (MCS; MW = 190,000–310,000 Da; 200–800 cP, 1% w/w in 1% acetic acid, 25 °C, Brookfield; 75–85% deacetylated), chitosan high molecular weight (HCS; MW = 310,000–375,000 Da; 800–2000 cP, 1% w/w in 1% acetic acid, 25 °C, Brookfield; >75% deacetylated), poly(vinyl alcohol) (PVA; MW = 9000–10,000 Da; 80% hydrolyzed), and Nile Red (NR) were purchased from Sigma Aldrich (Milan, Italy), sodium stearate (Na-SA) was obtained from AAKON Polichimica Srl (Milan, Italy), sodium behenate (Na-BA) was obtained from Larodan, Research Grade Lipids (Monroe, MO, USA), and sodium hydroxide (NaOH 0.1M) and hydrochloric acid (HCl 1M) were obtained from Carlo Erba Reagents Srl (Milan, Italy).
All reagents used for cell cultures were purchased from Sigma Aldrich (Milan, Italy).
For the synthesis of O-carboxymethyl chitosan (O-CMCS), all the reactants and solvents were supplied by Sigma Aldrich (Milan, Italy).
For the preparation of electrospun nanofibers, the following materials were used: Kolliphor P407 poloxamer (P407, BASF SE, Ludwigshafen, Germany) and poly(ethylene oxide) high molecular weight (h-PEO, MW = 4000 kDa, Colorcon, Dartford, UK).
All reagents used for cell cultures were purchased from Sigma Aldrich (Milan, Italy).
For the synthesis of O-carboxymethyl chitosan (O-CMCS), all the reactants and solvents were supplied by Sigma Aldrich (Milan, Italy).
For the preparation of electrospun nanofibers, the following materials were used: Kolliphor P407 poloxamer (P407, BASF SE, Ludwigshafen, Germany) and poly(ethylene oxide) high molecular weight (h-PEO, MW = 4000 kDa, Colorcon, Dartford, UK).