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Spectrum express software

Manufactured by PerkinElmer

Spectrum Express software is a laboratory analysis tool that enables data acquisition, processing, and reporting for a variety of spectroscopic techniques. The software provides a user-friendly interface for managing and analyzing spectroscopic data.

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2 protocols using spectrum express software

1

ATR-FTIR Analysis of Peptide Conjugates

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Attenuated total reflectance Fourier transform infrared (ATR-FTIR)
spectra were collected on a PerkinElmer Spectrum 100 spectrometer
(Waltham, MA, USA) equipped with a universal ATR sampling accessory.
Spectra were collected between 450 and 4000 cm–1 and processed using PerkinElmer Spectrum Express software. Peptide
conjugates were dissolved in 0.1 M HEPES for 1 day, and then, the
fibers were dialyzed in Nanopure H2O using a dialyzer mini
tube (Millipore, D-TubeTM, MWCO 12–14 kDa, catalog
no. 71505–3). The samples were concentrated by air evaporation.
1 μL of the concentrated samples was drop-cast onto ATR crystal
surface and allowed to air dry. The collected spectra were background-corrected
in air.
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2

Characterization of Functionalized PEEK Surfaces

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An attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectrometer (Spectrum 100, Perkin Elmer, Waltham, MA, USA) equipped with a Ge crystal ATR unit (VariGATR, Harrick Scientific Products, Pleasantville, NY, USA) was used to detect the characteristic functional groups on the bare PEEK, UV light-irradiated PEEK and MePRX-PEEK surfaces.
All the spectra were recorded in the frequency range of 1,000-4,000 cm -1 at a resolution of 4 cm -1 and analyzed using Spectrum Express software (PerkinElmer). Static contact angle analysis was performed in an air atmosphere using a contact angle measurement instrument (DropMaster DM-501, Kyowa Interface Science, Saitama, Japan) for four types of PEEK surfaces: bare, MePRX-VBn-coated, UV light-irradiated, and UV light-irradiated MePRX-VBn-coated PEEK surfaces. All samples were washed with DMSO and ethanol and then dried at 60°C. A single water droplet (5 μL) was placed on each surface and monitored using a charge-coupled device camera at 23°C. The captured images were analyzed using FAMAS version 5.0.0 software (Kyowa Interface Science).
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