β tubulin 3

Manufactured by Fortrea

β-tubulin III is a protein that is a component of microtubules, which are structural elements within cells. It plays a role in the organization and function of the cytoskeleton.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) Nestin, by Fortrea (1 mentions) Tuj1 clone, by Fortrea (1 mentions) H3k4me1, by Merck Group (1 mentions) H3k4me2, by Merck Group (1 mentions) Vinculin, by Merck Group (1 mentions) β tubulin, by Merck Group (1 mentions) Ha tag, by Abcam (1 mentions) Nestin, by Abcam (1 mentions) Phospho smad3, by Cell Signaling Technology (1 mentions) Smad2 3, by BD (1 mentions) Myc tag, by Abcam (1 mentions) Mms 435p, by Agilent Technologies (1 mentions) H3k27me2, by Cell Signaling Technology (1 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (1 mentions) H3k4me3, by Abcam (1 mentions) H3k27me3, by Merck Group (1 mentions) H3k27ac, by Abcam (1 mentions) Smad3, by Abcam (1 mentions) Ascl1, by BD (1 mentions)

3 protocols using β tubulin 3

Immunocytological Analysis of iPSCs and EBs

Cited 1 time

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Immunocytological analysis of iPSCs and embryoid bodies was as described previously [30 (link)]. Neural rosettes, NPs and neurons were fixed with 4% paraformaldehyde and stained with Pax6 (Covance), Nestin (Covance), MAP2 (AbCam) and β-tubulin III (TUJ1 clone; Covance) antibodies using standard methods.

Kim K.H., Liu J., Sells Galvin R.J., Dage J.L., Egeland J.A., Smith R.C., Merchant K.M, & Paul S.M. (2015). Transcriptomic Analysis of Induced Pluripotent Stem Cells Derived from Patients with Bipolar Disorder from an Old Order Amish Pedigree. PLoS ONE, 10(11), e0142693.

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Molecular Profiling of Stem Cell Differentiation

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TGFβ was acquired from Millipore (GF111). Antibodies used were anti: H3K27me3 (Millipore, 07449), H3K4me1 (Millipore, 07436), H3K4me2 (Millipore, 07030), H3K27me2 (Cell signaling, 9728S), H3K27ac (Abcam, ab4729), H3K4me3 (Abcam, ab8580), SMAD3 (Abcam, ab28379), phospho-SMAD3 (Cell Signaling, mAb9520), SMAD2/3 (BD Bioscience, 610842), ASCL1 (BD Pharmingen, 556604), JMJD3 (raised in the laboratory using amino acids 798–1095), CHD8 was raised in Dr José Carlos Reyes laboratory (32 (link)), β-TUBULIN III (TUJ1, Covance, MMS-435P), GFAP (Dako, z0334), NESTIN (Abcam, ab5968), HuC/D (MP, A21271), DAPI (ThermoFisher, D1306), β-TUBULIN (Millipore, MAB3408), VINCULIN (Sigma, V9131), HA tag (Abcam, ab20084) and MYC tag (Abcam, ab9132).

Fueyo R., Iacobucci S., Pappa S., Estarás C., Lois S., Vicioso-Mantis M., Navarro C., Cruz-Molina S., Reyes J.C., Rada-Iglesias Á., de la Cruz X, & Martínez-Balbás M.A. (2018). Lineage specific transcription factors and epigenetic regulators mediate TGFβ-dependent enhancer activation. Nucleic Acids Research, 46(7), 3351-3365.

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Immunostaining Tissue Sections: Antibody Panel

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Primary antibodies used to stain tissue sections were aPKC (#610207; Becton Dickinson), BrdU (#347580; BD and ab6326; Abcam), α-Catenin (610193; BD), Chx10 (X1179P; Exalpha Biologicals), CC3 (#9661; Cell Signaling Technology (CST)), Pan-Crb (synthesized using VGARVPPTPNLKLPPEERLI), Ezrin (ab4069; Abcam), GFP (GFP-1020; Aves), Lamin B1 (#9087; CST), Luciferase (ab21176; Abcam), Mitf (#x2398M; Exalpha), Otx2 (ab9566; Millipore), p27 (#610241; BD), Pals1 (07-708; Millipore), Pax2 (PRB-276P; Covance), Pax6 (RBP-278P; Covance), PCNA (#2586; CST), pH3 (#06-570; Millipore), rhodopsin (#1840-RHO; Phosphosolutions), Sox9 (AB5809; Millipore), β-Tubulin III (MMS-435P; Covance), Yap (#4912; CST and ab56701; Abcam), pYap (#4911; CST) and Zo1 (#610966; BD). Secondary antibodies used were Alexa488 conjugated anti-mouse (Life Tech.), chicken (Life Tech), rabbit (Life Tech) and rat (Life Tech) antibodies, Cy3 conjugated anti-rabbit and mouse antibodies (Jackson Immuno Res.) and Cy5 conjugated anti-mouse and rabbit antibodies (Life Tech.).

Kim J.Y., Park R., Lee J.H., Shin J., Nickas J., Kim S, & Cho S.H. (2016). Yap is essential for retinal progenitor cell cycle progression and RPE cell fate acquisition in the developing mouse eye. Developmental biology, 419(2), 336-347.

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