E myco pcr kit

Manufactured by iNtRON Biotechnology

The E-Myco PCR kit is a laboratory equipment product designed for the detection and identification of fungal species through the amplification of specific DNA sequences using the polymerase chain reaction (PCR) technique. The kit provides the necessary reagents and protocols to facilitate the PCR process, allowing for the rapid and accurate detection of fungal contaminants in various samples.

Automatically generated - may contain errors

Lab products found in correlation

Doxycycline hyclate, by Merck Group (1 mentions) Ht 29 luc2, by PerkinElmer (1 mentions) Nci h1373, by American Type Culture Collection (1 mentions) Nci h1838, by American Type Culture Collection (1 mentions) Du4475, by American Type Culture Collection (1 mentions) A2780, by American Type Culture Collection (1 mentions) Cal27, by American Type Culture Collection (1 mentions)

2 protocols using e myco pcr kit

Cell Line Characterization and Reagent Details

Check if the same lab product or an alternative is used in the 5 most similar protocols

FaDu and 293T cells were purchased from ATCC. LON-LICR-HN5 cell lines were from Prof. Sue Eccles (ICR, London, UK). Cells were cultured in DMEM, 5% FBS, 1% (v/v) glutamine, and 0.5% (v/v) penicillin/streptomycin. Short tandem repeat (STR) profiling was carried out by Bio-Synthesis. Mycoplasma testing used the e-Myco PCR kit (Intron Biotechnology). Experiments were carried out within 3 months of resuscitation. GSK2606414 was obtained from MedKoo Biosciences (NC, USA). Doxycycline hyclate was obtained from Sigma-Aldrich (Gillingham, UK). Reovirus type 3 Dearing (Reolysin/pelareorep) was kindly supplied by Oncolytics Biotech (Calgary, Canada).

McLaughlin M., Pedersen M., Roulstone V., Bergerhoff K.F., Smith H.G., Whittock H., Kyula J.N., Dillon M.T., Pandha H.S., Vile R., Melcher A.A, & Harrington K.J. (2020). The PERK Inhibitor GSK2606414 Enhances Reovirus Infection in Head and Neck Squamous Cell Carcinoma via an ATF4-Dependent Mechanism. Molecular Therapy Oncolytics, 16, 238-249.

+ Open protocol

+ Expand

Cell Line Provenance and Authentication

Check if the same lab product or an alternative is used in the 5 most similar protocols

CAL27, FaDu, A549 (obtained 2013), PJ34, PJ41 (obtained 2014), T24, A2780, RT4 (obtained 2012), NCI-H1838 (2013), NCI-H1373 (2015), DU-4475 (year unknown, STR profiled 2016) cell lines were purchased from ATCC. SCC090, and SCC131 were purchased from DSMZ (2014). SK-OV-3 (2011) was obtained from HPA culture collection (Porton Down). HCT116 p53 Xman isogenic cell lines were purchased from Horizon Discovery (2014), DLD1 BRCA2 wild-type and deficient cells were provided to the ICR as part of a collaborative agreement. HT-29-luc2 was purchased from Caliper (2015). LON-LICR-HN4 and LON-LICR-HN5 cell lines from Professor Sue Eccles (ICR, London, 2012). STR profiling was carried out by Bio-Synthesis Inc. Mycoplasma testing used the e-Myco PCR kit (Intron Biotechnology). Cell lines received from noncommercial sources were STR profiled prior to freezing, all cell lines were mycoplasma tested, and experiments were carried out within 3 months of resuscitation.

Dillon M.T., Barker H.E., Pedersen M., Hafsi H., Bhide S.A., Newbold K.L., Nutting C.M., McLaughlin M, & Harrington K.J. (2016). Radiosensitization by the ATR Inhibitor AZD6738 through Generation of Acentric Micronuclei. Molecular cancer therapeutics, 16(1), 25-34.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!