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Lathosterol

Manufactured by Avanti Polar Lipids
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Sourced in United States, Germany

Lathosterol is a laboratory product manufactured by Avanti Polar Lipids. It is a naturally occurring sterol that serves as a key intermediate in the biosynthesis of cholesterol.

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Lab products found in correlation

Desmosterol, by Avanti Polar Lipids (4 mentions) 2h6 lanosterol, by Avanti Polar Lipids (1 mentions) Lanosterol, by Avanti Polar Lipids (1 mentions) Zymosterol, by Avanti Polar Lipids (1 mentions) Campesterol, by Merck Group (1 mentions) Chloroform, by Carl Roth (1 mentions) Acetone, by Carl Roth (1 mentions) 2 hydroxyethylcellulose, by Merck Group (1 mentions) Stigmasterol, by Merck Group (1 mentions) Cholesterol, by Merck Group (1 mentions) Gelatin, by Merck Group (1 mentions)

4 protocols using lathosterol

1

Sterol Biosynthesis Pathway Enzymes

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DMHCA, lanosterol, [26,26,26,27,27,27-2H6]lanosterol, zymosterol, [2,2,3,4,4,-2H5]zymosterol, lathosterol, and desmosterol were from Avanti Polar Lipids, Inc. (Alabaster, AL, United States). Cholesterol and [3H]cholesterol were from Steraloids, Inc. (Newport, RI, United States) and PerkinElmer (Waltham, MA, United States), respectively. [26,26,26,27,27,27-2H6]desmosterol and [1,2,5,6α-2H4]lathosterol were from C/D/N Isotopes Inc. (Pointe-Claire, QC, Canada). Rodent chow (5P76 Prolab Isopro RMH 3000) was from T. R. Last Co. (Saxonburg, PA, United States). All other chemicals were from Sigma-Aldrich (St. Louis, MO, United States) unless otherwise indicated. Recombinant CYP27A1, CYP46A1, adrenodoxin reductase, adrenodoxin, and NADPH-CYP oxidoreductase were expressed and purified as described (Sagara et al., 1992 (link), 1993 (link); Hanna et al., 1998 (link); Mast et al., 2006 (link), 2012 (link)).
El-Darzi N., Astafev A., Mast N., Saadane A., Lam M, & Pikuleva I.A. (2018). N,N-Dimethyl-3β-hydroxycholenamide Reduces Retinal Cholesterol via Partial Inhibition of Retinal Cholesterol Biosynthesis Rather Than its Liver X Receptor Transcriptional Activity. Frontiers in Pharmacology, 9, 827.
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2

Embedding Medium Preparation Protocol

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Chloroform, methanol (MeOH), acetonitrile (ACN), and acetone were from Roth (Karlsruhe, Germany); 2,5-dihydroxyacetophenone (DHAP) was from Merck (Darmstadt, Germany); lathosterol and desmosterol were from Avanti Polar Lipids (Alabaster, AL, USA); and campesterol, cholesterol, and stigmasterol were from Sigma-Aldrich (Merck, Darmstadt, Germany). Embedding medium, prepared according to Nelson et al. [46 (link)], consisted of 5% 2-hydroxyethyl cellulose (Mv,avg ~ 90,000; Merck) and 10% gelatin (Merck) in ultrapure water.
Bien T., Hambleton E.A., Dreisewerd K, & Soltwisch J. (2020). Molecular insights into symbiosis—mapping sterols in a marine flatworm-algae-system using high spatial resolution MALDI-2-MS imaging with ion mobility separation. Analytical and Bioanalytical Chemistry, 413(10), 2767-2777.
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3

Quantitative Sterol Profiling by GC-MS

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GC-MS analysis was performed using MassHunter software. Identification of TMS ethers of natural sterols was determined through comparison to commercially available standards for cholesterol, 7DHC, lathosterol, and desmosterol (Avanti Polar Lipids, Inc.), as well as comparison to MS spectra through the National Institute of Standards and Technologies Standard Reference Database when available. Identification of 8DHC was inferred as an isomer of 7DHC and comparison to SLOS fibroblasts; zymostenol identification was based on spectra from Conradi-Hünermann-Happle syndrome (CDPXD2) fibroblasts. Retention times and mass to charge (m/z) ratios are summarized in Table S1. Representative MS fragmentation patterns available upon request. TMS derivatives of sterols exhibiting abundance < 3% were excluded from analysis. For peak quantitation, sterol abundance was normalized to both the internal standard (coprostanol) and protein concentration. Data is presented relative to control samples using GraphPad Prism software.
Anderson R.H., Sochacki K.A., Vuppula H., Scott B.L., Bailey E.M., Schultz M.M., Kerkvliet J.G., Taraska J.W., Hoppe A.D, & Francis K.R. (2021). Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis. Cell reports, 37(7), 110008.
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4

Quantitative Sterol Profiling by GC-MS

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GC-MS analysis was performed using MassHunter software. Identification of TMS ethers of natural sterols was determined through comparison to commercially available standards for cholesterol, 7DHC, lathosterol, and desmosterol (Avanti Polar Lipids, Inc.), as well as comparison to MS spectra through the National Institute of Standards and Technologies Standard Reference Database when available. Identification of 8DHC was inferred as an isomer of 7DHC and comparison to SLOS fibroblasts; zymostenol identification was based on spectra from Conradi-Hünermann-Happle syndrome (CDPXD2) fibroblasts. Retention times and mass to charge (m/z) ratios are summarized in Table S1. Representative MS fragmentation patterns available upon request. TMS derivatives of sterols exhibiting abundance < 3% were excluded from analysis. For peak quantitation, sterol abundance was normalized to both the internal standard (coprostanol) and protein concentration. Data is presented relative to control samples using GraphPad Prism software.
Anderson R.H., Sochacki K.A., Vuppula H., Scott B.L., Bailey E.M., Schultz M.M., Kerkvliet J.G., Taraska J.W., Hoppe A.D, & Francis K.R. (2021). Sterols lower energetic barriers of membrane bending and fission necessary for efficient clathrin-mediated endocytosis. Cell reports, 37(7), 110008.
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