HCT-116 (colorectal carcinoma), HT-29 (colorectal adenocarcinoma) and MDA-MB-231 (breast adenocarcinoma) cell lines were purchased from American Type Culture Collection (ATCC). MDA-MB-231 cells were grown in RPMI media while HCT-116 and HT-29 cells were cultured in McCoy's 5A media, both supplemented with 10% FBS and antibiotics for 24 h before treatment with specified compounds for indicated times. Authentication of cell lines was done by ATCC through their DNA-STR profile. CDK1/Cyclin B1, CDK2/Cyclin A2, CDK4/Cyclin D1, Retinoblastoma (C-term) and kinase buffer were purchased from SignalChem. Aspirin, salicylic acid and trypsin-EDTA solution were purchased from Sigma-Aldrich; H1 Histones were obtained from EMD Millipore; [32P] γ-ATP from PerkinElmer; 2,3-DHBA, 2,5-DHBA and all other reagents mentioned in this study were obtained from Thermo Fischer Scientific, Inc.
Cdk2 cyclin a2
Manufactured by Sino Biological
Sourced in Canada
CDK2/Cyclin A2 is a recombinant protein that consists of the catalytic subunit CDK2 and the regulatory subunit Cyclin A2. It is an important component in the regulation of the cell cycle and plays a key role in the transition from the G1 phase to the S phase.
Automatically generated - may contain errors
Lab products found in correlation
Kinase buffer, by Sino Biological (2 mentions)
Cdk4 cyclin d1, by Sino Biological (2 mentions)
Cdk1 cyclin b1, by Sino Biological (2 mentions)
Retinoblastoma c term, by Sino Biological (2 mentions)
Aspirin, by Merck Group (1 mentions)
Salicylic acid, by Merck Group (1 mentions)
2 5 dhba, by Thermo Fisher Scientific (1 mentions)
Trypsin edta solution, by Merck Group (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
Hct116, by American Type Culture Collection (1 mentions)
γ 32p atp, by PerkinElmer (1 mentions)
Anti p21cip1, by Cell Signaling Technology (1 mentions)
Anti cdk2, by Cell Signaling Technology (1 mentions)
Anti cdk4, by Cell Signaling Technology (1 mentions)
Anti cyclin d1, by Cell Signaling Technology (1 mentions)
Anti cyclin b1, by Cell Signaling Technology (1 mentions)
Anti cdk1, by Cell Signaling Technology (1 mentions)
Anti p27kip1, by Cell Signaling Technology (1 mentions)
Anti cyclin a2, by Cell Signaling Technology (1 mentions)
Anti β tubulin, by Cell Signaling Technology (1 mentions)
2 protocols using cdk2 cyclin a2
1
Colorectal and Breast Cancer Cell Line Cultivation and Kinase Assay
2
Protein Expression and Kinase Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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anti-p21Cip1, anti-p27Kip1, anti-CDK1, anti-CDK2, anti-CDK4, anti-cyclinA2, anti-cyclinB1, anti-cyclinD1, and anti-β tubulin antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA); anti-SLC5A8 from Invitrogen (Carlsbad, CA, USA); goat anti-rabbit and goat anti-mouse antibodies were obtained from Bio-Rad (Hercules, CA, USA). CDK1/Cyclin B1, CDK2/Cyclin A2, CDK4/Cyclin D1, Retinoblastoma (C-term) and kinase buffer were purchased from SignalChem (Richmond, BC, Canada).
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