Human 293T cell lines were acquired from the American Type Culture Collection (ATCC). Cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) complete medium containing glucose and L-glutamine (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Invitrogen).
Human 293t cell lines
Manufactured by American Type Culture Collection
Sourced in United States
The Human 293T cell line is a widely used cell line derived from human embryonic kidney cells. It is a transfection-permissive line that expresses the SV40 large T antigen. The 293T cell line is commonly used for the production of recombinant proteins and viral vectors.
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2 protocols using human 293t cell lines
1
Human 293T Cell Culture
2
Culturing Human Glioblastoma Cell Lines
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The human GBM cell lines T98, A172, U87 (glioblastoma of unknown origin; STR profiling was performed), LN229, U251 and U118 (derived from the U138MG astrocytoma cell line; STR profiling was performed) were obtained from the Chinese Academy of Sciences Cell Bank (Shanghai, China). All cells were cultured in Dulbecco's modified Eagle's medium (DMEM) with high glucose and sodium pyruvate, supplemented with 10% fetal bovine serum (FBS) and antibiotics (100 U/ml penicillin and 100 ng/ml streptomycin). Cells were maintained in a 5% CO2 atmosphere at 37°C. Normal human astrocytes (NHAs) were purchased from Lonza (Walkersville, MD, USA) and cultured according to the supplier's instructions. Human 293T cell lines were purchased from the American Type Culture Collection (ATCC; Rockville, MD, USA).
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