Estradiol hemihydrate

The experiment was carried out on three-month-old female Wistar rats. The experiment was conducted under the approval of the Local Ethics Committee in Katowice (permission numbers: 38/2015, 148/2015, and 66/2016). The rats were purchased at the Center of Experimental Medicine, Medical University of Silesia (Katowice, Poland).
In the course of the experiment the following drugs were administered orally to the rats: rosmarinic acid (Sigma-Aldrich, St. Louis, MO, USA), sinapic acid (Sigma-Aldrich, St. Louis, MO, USA) and estradiol hemihydrate (Estrofem, Novo Nordisk A/S, Bagsvard, Denmark). As anesthetics ketamine (Ketamina 10%, Biowet Puławy, Puławy, Poland) and xylazine (Xylapan, Vetoquinol Biowet, Gorzów Wlkp., Poland) were used.

The experiment was conducted on mature, 3-month-old female Wistar rats, with the approval of the Local Ethics Commission in Katowice (permission numbers 38/2015, 148/2015, and 66/2016). The animals were provided by the Center of Experimental Medicine, Medical University of Silesia, Katowice, Poland. Rats had unlimited access to drinking water and standard laboratory feed (Labofeed B; Wytwórnia Pasz “Morawski”, Kcynia, Poland). The following substances and drugs were used: sinapic acid (Sigma-Aldrich, St. Louis, MO, USA), estradiol hemihydrate (Estrofem, Novo Nordisk A/S, Bagsvard, Denmark), ketamine (Ketamina 10%, Biowet Puławy, Puławy, Poland), xylazine (Xylapan, Vetoquinol Biowet, Gorzów Wlkp., Poland).