Cisbio ip one kit

Because the lifetime of IP 3 within the cell is very short before it is transformed into IP 2 and IP 1 , IP 1 accumulation levels can be used to represent IP 3 levels in cells [32] . The IP 1 measurements were performed as recently described [32] . Agonist-induced IP 1 in LiCl-treated cells was quantified using the Cisbio IP-One kit (Cisbio Bioassays, Codolet, France) per the manufacturer's instructions. Light emitted at 615 and 665 nm was measured on a Spectra Max i3X Multimode Plate Reader (Molecular Devices, CA, USA) using the HTRF mode after excitation at 340 nm. The FRET ratios (665 nm/615 nm) were converted to concentrations of IP 1 using the provided standard.