Amicon ultra 15 100 kda centrifugal filter devices

Manufactured by Merck Group

The Amicon Ultra-15 100-kDa Centrifugal Filter Devices are a type of lab equipment used for the concentration and desalting of protein samples. The devices feature a 100-kDa molecular weight cutoff membrane that allows the passage of small molecules while retaining larger proteins during the centrifugation process.

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Lab products found in correlation

Centricon plus 70 100 kda centrifugal filter devices, by Merck Group (2 mentions) Boric acid, by Thermo Fisher Scientific (1 mentions) Trizma ph 9, by Merck Group (1 mentions)

Close spelling variants of this product

Amicon 100 KDa Ultra-15 centrifugal devices Amicon Ultra-15 (100 kDa) filter devices Amicon Ultra-15 Centrifugal Filter Devices 100 kDa centrifugal filter device Amicon Ultra centrifugal filter devices Amicon Ultra-15 Filter Devices Amicon® Ultra-15-100 kDa Amicon centrifugal filter devices Amicon®Ultra-15 device Amicon Ultra 100 kDa

2 protocols using amicon ultra 15 100 kda centrifugal filter devices

Concentrating Cell Culture Supernatants

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Inactivated cell culture supernatants were concentrated in Amicon Ultra-15 100-kDa Centrifugal Filter Devices (Millipore) or Centricon Plus-70 100-kDa Centrifugal Filter Devices at 3500 x g for 10–45 min at 4°C. Any material that was inadvertently concentrated beyond the desired concentration factor was diluted to the correct volume with 0.1M trizma/BS buffer or 1X PBS.

Goodman C.H., Russell B.J., Velez J.O., Laven J.J., Nicholson W.L., Bagarozzi DA J.r., Moon J.L., Bedi K, & Johnson B.W. (2014). Development of an algorithm for production of inactivated arbovirus antigens in cell culture. Journal of virological methods, 208, 66-78.

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Antigen Concentration and Inactivation

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Antigen was concentrated after inactivation, as it had been determined empirically that antigen activity was lost if it was concentrated before inactivation. Inactivated cell culture supernatants were concentrated in Amicon Ultra-15 100kDa Centrifugal Filter Devices (Millipore, Billerica, MA) or Centricon Plus-70 100-kDa Centrifugal Filter Devices (Millipore) at 3500 x g for 10-45 min at 4°C. The final volume was adjusted with 0.1M trizma/BS buffer: 1.0M trizma pH 9.0 (Sigma-Aldrich) + borate saline solution pH 9.0 [1.5M sodium chloride (Daigger, Vernon Hills, IL), 0.5M boric acid (Fisher Scientific), 1.0N sodium hydroxide (Daigger)] to the desired concentration factor.

Goodman C.H., Russell B.J., Velez J.O., Laven J.J., Bagarozzi DA J.r., Moon J.L., Bedi K, & Johnson B.W. (2015). Production of a Sindbis/Eastern Equine Encephalitis Chimeric Virus Inactivated Cell Culture Antigen. Journal of virological methods, 223, 19-24.

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