Cd16 apcvio770

Manufactured by Miltenyi Biotec

The CD16-APCVio770 is a fluorochrome-conjugated antibody that recognizes the CD16 antigen. CD16 is a low-affinity Fc receptor, also known as FcγRIII, which is expressed on natural killer cells, macrophages, and a subset of T cells. The CD16-APCVio770 antibody can be used for the identification and analysis of CD16-positive cell populations in flow cytometry applications.

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Lab products found in correlation

Cd56 pe vio770, by Miltenyi Biotec (1 mentions) Cd3 apc cy7 clone sk7, by BioLegend (1 mentions) Propidium iodide, by Miltenyi Biotec (1 mentions) Cd3 apc vio770, by Miltenyi Biotec (1 mentions) Macsquant analyzer, by FlowJo (1 mentions) Cd20 pe vio770, by Miltenyi Biotec (1 mentions) Live dead aqua, by Thermo Fisher Scientific (1 mentions) Cd8 percp vio700, by Miltenyi Biotec (1 mentions) Cd69 vioblue, by Miltenyi Biotec (1 mentions) Cd4 pe, by BD (1 mentions)

2 protocols using cd16 apcvio770

Comprehensive Immune Cell Profiling

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α-CD2-PerCP-Vio770 (clone LT2, Miltenyi Biotec), NFL1 viability dye (OncoImmunin), CD56-BV421 (clone HCD56, Biolegend), CD2-BV510 (clone RPA-2.10, Biolegend), CD4-PEVio770 (clone REA623, Miltenyi Biotec), CD45RA-APC (clone REA562, Miltenyi Biotec), CD3-APC/Cy7 (clone SK7, Biolegend), propidium iodide (Miltenyi Biotec), CCR7-BV421 (clone 3D12, BD Biosciences), CD27-PE (clone REA499, Miltenyi Biotec), CD16-APCVio770 (clone REA426, Miltenyi Biotec) and CD56-PEVio770 (clone REA196, Miltenyi Biotec).

Tomalka A.G., Resto-Garay I., Campbell K.S, & Popkin D.L. (2018). In vitro Evidence That Combination Therapy With CD16-Bearing NK-92 Cells and FDA-Approved Alefacept Can Selectively Target the Latent HIV Reservoir in CD4+ CD2hi Memory T Cells. Frontiers in Immunology, 9, 2552.

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Multicolor Flow Cytometry for Immune Cell Phenotyping

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Flow cytometric analyses of frequencies of activated B cells, T cells, and monocytes were performed using EDTA anticoagulated blood predose and at 24 h postdose. The following antibody cocktail was used for B and T cell phenotyping: CD3 APC-Vio770 (130-104-236; Miltenyi), CD4 PE (550630; BD Biosciences), CD8 PerCp-Vio700 (130-097-911; Miltenyi), CD20 PE-Vio770 (130-099-733; Miltenyi), CD69 VioBlue (130-106-596; Miltenyi), CD25 BB15 (565096; BD Biosciences), and Live/Dead Aqua (1737195; Molecular Probes). The following antibody cocktail was used for monocyte phenotyping: CD14 PE-Cy7 (130-091-242; Miltenyi), CD16 APC Vio770 (130-106-765; Miltenyi), and CD169-APC (346008; BD Biosciences). Data (150,000 events) were acquired on a MACSQuant analyzer 10 and analyzed by FlowJo software.

Bekerman E., Hesselgesser J., Carr B., Nagel M., Hung M., Wang A., Stapleton L., von Gegerfelt A., Elyard H.A., Lifson J.D, & Geleziunas R. (2019). PD-1 Blockade and TLR7 Activation Lack Therapeutic Benefit in Chronic Simian Immunodeficiency Virus-Infected Macaques on Antiretroviral Therapy. Antimicrobial Agents and Chemotherapy, 63(11), e01163-19.

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