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Cary 5espectrophotometer

Manufactured by Agilent Technologies
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The Cary 5e Spectrophotometer is a high-performance UV-Vis-NIR spectrophotometer designed for a wide range of analytical applications. It measures the absorption or transmission of light in the ultraviolet, visible, and near-infrared regions of the electromagnetic spectrum.

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Lab products found in correlation

J 715 spectropolarimeter, by Oxford Instruments (1 mentions) Sm 4000 8t magnetocryostat, by Oxford Instruments (1 mentions) Ft ir 410, by Jasco (1 mentions) Ft ir 6300 spectrometer, by Jasco (1 mentions)

3 protocols using cary 5espectrophotometer

1

Spectroscopic Characterization of Samples

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Room-temperature electronic
absorption (RT Abs) spectra were collected using a Varian Cary 5e
spectrophotometer. Low-temperature (LT) Abs and MCD spectra were collected
with a Jasco J-715 spectropolarimeter in conjunction with an Oxford
Instruments SM4000-8T magnetocryostat. To reduce contributions from
the CD background signal and glass strain, the MCD spectra presented
herein were obtained by taking the difference between spectra obtained
with the magnetic field aligned parallel and antiparallel to the light-propagation
axis. Iterative Gaussian deconvolutions of all absorption and MCD
spectra were conducted using IGOR version 6.22a.27 X-band EPR spectra were collected using a Bruker
ESP 300E spectrometer equipped with a Varian EIP model 625A continuous-wave
frequency counter. The sample temperature was maintained at 20 K by
an Oxford ESR 900 continuous-flow liquid He cryostat that was regulated
by an Oxford ITC temperature controller. Spectra were fit using the
EasySpin program by Stoll and Schweiger.28 (link) Double integration of the signals was conducted using IGOR version
6.22a.27
Blaesi E.J., Fox B.G, & Brunold T.C. (2014). Spectroscopic and Computational Investigation of Iron(III) Cysteine Dioxygenase: Implications for the Nature of the Putative Superoxo-Fe(III) Intermediate. Biochemistry, 53(36), 5759-5770.
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2

Detailed Spectroscopic Analysis of Samples

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X-band EPR spectra were collected on a Bruker ESP 300E spectrometer equipped with an Oxford ESR 900 continuous-flow liquid helium cryostat and an Oxford ITC4 temperature controller. All spectra were collected at 20 K using the following instrument settings: frequency = 9.38 GHz, microwave power = 2.0 mW, gain = 40–60 dB, modulation amplitude = 5 G, conversion time = 87 ms, time constant = 327.68 ms, range = 600–4598 G, resolution = 2048 points and modulation frequency = 100kHz. Simulated EPR spectra were fitted using the SIMPOW program written by Dr. Mark Nilges.38 Room temperature Abs spectra were obtained using a Varian Cary 5e spectrophotometer. The sample compartment was purged with N2(g) for 40 min prior to data collection. Frozen solution Abs and MCD spectra were collected with a JASCO J-715 spectropolarimeter in conjunction with an Oxford Instruments SM-4000 8 T superconducting magnetocryostat. Circular dichroism and glass-strain contributions to the MCD spectra were removed by taking the difference between spectra obtained with the magnetic field oriented parallel and anti-parallel to the light of propagation axis. Sample conditions are listed in the caption of each figure.
Stracey N.G., Costa F.G., Escalante-Semerena J.C, & Brunold T.C. (2018). Spectroscopic Study of the EutT Adenosyltransferase from Listeria monocytogenes: Evidence for the Formation of a Four-Coordinate Cob(II)alamin Intermediate.. Biochemistry, 57(34), 5088-5095.
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3

Comprehensive Material Characterization

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Analytical data were obtained in a Thermo-Scientific (Flash 2000) elemental micro-analyzer. FT-IR spectra were recorded using KBr pellets on a Jasco FT-IR 410 or a Jasco FT-IR 6300 spectrometer. Thermogravimetric (TG) analyses were carried out in air-dry flow (100 ml/min) by a Shimadzu thermo-balance TGA-DTG-50H instrument, coupled with a FT-IR Nicolet Magma 550 spectrometer. A series of FT-IR spectra (~20-30 per sample) of the evolved gasses were time-spaced recorded during the TG experiment. Diffuse reflectance (electronic) spectra were recorded in a Varian Cary-5E spectrophotometer.
González-Pérez J.M., Choquesillo-Lazarte D., Domínguez-Martín A., El Bakkali H., García-Rubiño M.E., Pérez-Toro I., Vílchez-Rodríguez E., Castiñeiras A., Nurchi V.M, & Niclós-Gutiérrez J. (2015). Molecular recognition between adenine or 2,6-diaminopurine and copper(II) chelates with N,O2,S-tripodal tetradentate chelators having thioether or disulfide donor groups. Journal of inorganic biochemistry, 151.
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