Attenuation‐based microtomographic images were acquired using a Zeiss Xradia 520 Versa 3D X‐ray microscope, with the x‐ray tube voltages source set at 80/7 or 40/3 keV/W (keV: kiloelectron volts). A thin LE1 filter was used to avoid beam‐hardening artifacts. During the CT scanning, the sample was placed on a rotation table and projection images were acquired over an angular range of 180 degrees. To obtain high resolution images, a CCD optical objective with 4× was applied in the scan. Images were acquired with voxel (volumetric 3D pixels after reconstruction) sizes of 1‐1.5 μm, and tomographic reconstructions were made with the resident software XMReconstructer (Carl Zeiss X‐ray Microscopy Inc., Pleasanton, CA). Reconstructed images were exported as TIFF and loaded into Avizo version 9.5 (Thermo Fisher Scientific, https://www.fei.com/software/amira-avizo/ ) for 3D visualization.
Avizo version 9
Manufactured by Thermo Fisher Scientific
Avizo version 9.5 is a comprehensive software suite for 3D data visualization and analysis. It provides a range of tools for processing, segmenting, and analyzing complex data from various scientific and industrial applications.
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Lab products found in correlation
3 protocols using avizo version 9
1
High-Resolution X-Ray Microtomography Imaging
2
High-Resolution X-Ray Microtomography Imaging
3
Microtomographic Imaging of Samples
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Attenuation-based microtomographic images were acquired using an Xradia 520 Versa 3D X-ray microscope (Zeiss), with the x-ray tube voltages source set at 80/7 or 40/3 keV/W (keV: kiloelectron volts). A thin LE1 filter was used to avoid beamhardening artifacts. During the CT scanning, the sample was placed on a rotation table and projection images were acquired over an angular range of 180 degrees. To obtain high resolution images, a 4x CCD (charge-coupled device) optical objective was used. Images were acquired with voxel sizes (volumetric 3D pixels after reconstruction) of 1-1.5 µm, and tomographic reconstructions were made with the resident software (XMReconstructor). Reconstructed images were exported as TIFF files and loaded into Avizo version 9.5 (Thermo Fisher Scientific) for 3D visualization.
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