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Qscript synthesis reagent

Manufactured by Quanta Biosciences
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QScript synthesis reagent is a kit that contains the necessary components for the reverse transcription of RNA into cDNA, which can then be used for downstream applications such as PCR or sequencing. The reagent includes a thermostable reverse transcriptase enzyme, buffer, and dNTPs. This product is intended for use in research laboratories.

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Lab products found in correlation

Sybr green master mix, by Qiagen (2 mentions) Quantstudio 3, by Thermo Fisher Scientific (2 mentions) Rneasy kit, by Qiagen (2 mentions)

Close spelling variants of this product

QScript™ Reagent

2 protocols using qscript synthesis reagent

1

RNA Extraction and qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was collected from cells using an RNeasy kit (Qiagen, 74004). RNA concentrations were measured using a NanoDrop spectrophotometer, and cDNA was synthesized with qScript synthesis reagent (Quanta Biosciences, 95048). qPCR was run with the primers shown in Table S2 using SYBR-Green Mastermix (SA Biosciences, 4385610) and an Applied Biosystems QuantStudio 3. During analysis, threshold cycle numbers were normalized to GAPDH or Actin levels.
Lauko A., Volovetz J., Turaga S.M., Bayik D., Silver D.J., Mitchell K., Mulkearns-Hubert E.E., Watson D.C., Desai K., Midha M., Hao J., McCortney K., Steffens A., Naik U., Ahluwalia M.S., Bao S., Horbinski C., Yu J.S, & Lathia J.D. (2022). SerpinB3 drives cancer stem cell survival in glioblastoma. Cell reports, 40(11), 111348.
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2

RNA Extraction and qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
RNA was collected from cells using an RNeasy kit (Qiagen, 74004). RNA concentrations were measured using a NanoDrop spectrophotometer, and cDNA was synthesized with qScript synthesis reagent (Quanta Biosciences, 95048). qPCR was run with the primers shown in Table S2 using SYBR-Green Mastermix (SA Biosciences, 4385610) and an Applied Biosystems QuantStudio 3. During analysis, threshold cycle numbers were normalized to GAPDH or Actin levels.
Lauko A., Volovetz J., Turaga S.M., Bayik D., Silver D.J., Mitchell K., Mulkearns-Hubert E.E., Watson D.C., Desai K., Midha M., Hao J., McCortney K., Steffens A., Naik U., Ahluwalia M.S., Bao S., Horbinski C., Yu J.S, & Lathia J.D. (2022). SerpinB3 drives cancer stem cell survival in glioblastoma. Cell reports, 40(11), 111348.
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