Log In Sign up
The largest database of trusted experimental protocols

Risc free co sirna

Manufactured by Horizon Discovery
Visit Supplier
Sourced in United States

RISC-free Co-siRNA is a laboratory reagent designed for targeted gene silencing. It functions by delivering small interfering RNA (siRNA) molecules to cells in a RISC-independent manner, allowing for efficient gene knockdown without the need for RISC complex formation.

Automatically generated - may contain errors

Lab products found in correlation

Siglo red transfection indicator, by Horizon Discovery (2 mentions) On targetplus, by Horizon Discovery (2 mentions) Silentfect, by Bio-Rad (2 mentions)

Close spelling variants of this product

RISC-free siRNA SiRNAs

2 protocols using risc free co sirna

1

siRNA Knockdown of NOTCH3 in CLL

Check if the same lab product or an alternative is used in the 5 most similar protocols
The siRNA duplexes (siRNAs) for NOTCH3 (ON-TARGETplusTM) and the controls (RISC-free Co-siRNA, and siGLO red transfection indicator) were obtained from Dharmacon (Lafayette, CO, USA). Transfection of siRNAs into the CLL cells was performed by using the lipid reagent siLentFectTM from Bio-Rad Laboratories (Hercules, CA, USA). The transfection efficiency was determined by FACS and varied from 70 to 90%.
Hubmann R., Schnabl S., Araghi M., Schmidl C., Rendeiro A.F., Hilgarth M., Demirtas D., Ali F., Staber P.B., Valent P., Zielinski C., Jäger U, & Shehata M. (2020). Targeting Nuclear NOTCH2 by Gliotoxin Recovers a Tumor-Suppressor NOTCH3 Activity in CLL. Cells, 9(6), 1484.
+ Open protocol
+ Expand
2

siRNA Knockdown of NOTCH2 in HCC

Check if the same lab product or an alternative is used in the 5 most similar protocols
siRNA duplexes (siRNAs) for NOTCH2 (ON-TARGETplusTM) and controls (RISC-free Co-siRNA, and siGLO red transfection indicator) were obtained from Dharmacon (Lafayette, CO, United States). Transfection of siRNAs into HCC cell lines was performed by using the lipid reagent siLentFectTM from Bio-Rad Laboratories. The transfection efficiency was determined by FACS and varied from 80 to 90%.
Hubmann R., Sieghart W., Schnabl S., Araghi M., Hilgarth M., Reiter M., Demirtas D., Valent P., Zielinski C., Jäger U, & Shehata M. (2017). Gliotoxin Targets Nuclear NOTCH2 in Human Solid Tumor Derived Cell Lines In Vitro and Inhibits Melanoma Growth in Xenograft Mouse Model. Frontiers in Pharmacology, 8, 319.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!