Both IBV Beaudette strain and IBV M41 strain (standard strain) were from our laboratory. For WB and immunofluorescence assays, the Flag-tag mouse monoclonal antibody (Beyotime Biotechnology), HRP-labeled goat anti-mouse IgG(H + L) (Beyotime Biotechnology), GAPDH Mouse Monoclonal Antibody (Beyotime Biotechnology), and Alexa Fluor 350 labeled goat anti-rabbit IgG (H + L) (Beyotime Biotechnology) were used as primary and second antibodies, respectively.
Gapdh mouse monoclonal antibody
The GAPDH Mouse Monoclonal Antibody is a laboratory reagent used to detect and quantify the presence of the GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) protein in biological samples. This antibody is specific to the GAPDH protein and can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and ELISA, to analyze the expression levels of GAPDH in cells or tissues.
Lab products found in correlation
6 protocols using gapdh mouse monoclonal antibody
Chicken Cell Lines for IBV Studies
Both IBV Beaudette strain and IBV M41 strain (standard strain) were from our laboratory. For WB and immunofluorescence assays, the Flag-tag mouse monoclonal antibody (Beyotime Biotechnology), HRP-labeled goat anti-mouse IgG(H + L) (Beyotime Biotechnology), GAPDH Mouse Monoclonal Antibody (Beyotime Biotechnology), and Alexa Fluor 350 labeled goat anti-rabbit IgG (H + L) (Beyotime Biotechnology) were used as primary and second antibodies, respectively.
Comprehensive Protein Analysis Pipeline
HSYA Isolation and Analysis in C. tinctorius
Western Blot Analysis of Apoptosis Proteins
Antibodies used for Western blots were as follows: SERPINB2 rabbit polyclonal antibody (ABclonal, A15297, Wuhan, China), GAPDH mouse monoclonal antibody (Beyotime Biotechnology, AF0006), Caspase-3 rabbit polyclonal antibody (Beyotime Biotechnology, AF0081). Secondary antibodies were HRP-conjugated goat anti-mouse IgG (Beyotime Biotechnology, A0216) and peroxidase-conjugated goat anti-rabbit IgG (Beyotime Biotechnology, A0208).
Protein Quantification and Western Blotting Protocol
Western Blot Protein Quantification Protocol
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