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Anti ki67 pe b56

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Anti-Ki67-PE (B56) is a fluorescent antibody that binds to the Ki-67 protein, a cellular marker of proliferation. It is commonly used in flow cytometry and immunohistochemistry applications to detect and quantify the presence of proliferating cells.

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Lab products found in correlation

Facsjazz, by BD (1 mentions) Anti cd4 apc rpa t4, by BioLegend (1 mentions) Anti cd45 pe hi30, by BioLegend (1 mentions) Anti cd3 apc cy7 hit3a, by BioLegend (1 mentions) Facscalibur, by BD (1 mentions) Cellquest software, by BD (1 mentions) Celltac α mek 6450, by Nihon Kohden (1 mentions) Anti hla dr apc clone g46 6, by BD (1 mentions) Facsverse flow cytometer, by BD (1 mentions) Percp cy5.5 anti cd4 clone okt4, by BioLegend (1 mentions) Fixation and permeabilization solution, by BD (1 mentions) Perm wash buffer, by BD (1 mentions) Lysing buffer, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Magnetic bead, by Miltenyi Biotec (1 mentions) Anti pd 1 pe ebioj105, by Thermo Fisher Scientific (1 mentions) Streptavidin pe cy7, by BioLegend (1 mentions)

Spelling variants of this product

Anti-Ki67 (102 citations) Ki67 (B56) (19 citations) Anti-Ki67 (B56, (13 citations) Anti-Ki67-PE (10 citations) Ki67- PE (B56, (4 citations) PE Ki67 (4 citations) Anti-human Ki67-PE (clone B56) (2 citations)

4 protocols using anti ki67 pe b56

1

Flow Cytometry and Hematometry Protocol for Cell Analysis

Cited 2 times
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Flow cytometry was performed with FACS Calibur (BD Biosciences) and FACSJazz (BD Biosciences) as previously described [11 (link),12 (link),22 (link)–24 (link)], and the obtained data were analysed with Cell Quest software (BD Biosciences) and FlowJo software (Tree Star, Inc.). For flow cytometry analysis, anti-CD45-PE (HI30; Biolegend), anti-CD3-APC-Cy7 (HIT3a; Biolegend), anti-CD4-APC (RPA-T4; Biolegend), anti-CD25-APC (BC96; eBioscience), and anti-Ki67-PE (B56; BD Biosciences) antibodies were used. Hematometry was performed with a Celltac α MEK-6450 (Nihon Kohden Co.) as previously described [11 (link),12 (link),23 (link),24 (link),49 (link)]. Live cell sorting was performed using FACSJazz (BD Biosciences) according to the manufacture's procedure. The purity of each population was >94% (see also S7 Fig).
Nakano Y., Misawa N., Juarez-Fernandez G., Moriwaki M., Nakaoka S., Funo T., Yamada E., Soper A., Yoshikawa R., Ebrahimi D., Tachiki Y., Iwami S., Harris R.S., Koyanagi Y, & Sato K. (2017). HIV-1 competition experiments in humanized mice show that APOBEC3H imposes selective pressure and promotes virus adaptation. PLoS Pathogens, 13(5), e1006348.
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2

Multiparameter Flow Cytometric Analysis of T Cells

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Multiparameter flow cytometry was performed using peripheral blood, as described previously (Ma et al., 2012 (link); Xia et al., 2009 (link); Zhang et al., 2017b (link); Zheng et al., 2014a (link)). For the frequency of HLA-DR+CD8+ T cells, blood was treated with lysing buffer (BD Biosciences, CA, USA) for 10 min and then incubated with a mixture of flow cytometry antibodies at 4 °C for 30 min. For staining with ki67, surface-labeled cells were further treated with fixation and permeabilization solution (BD Biosciences, CA, USA), followed by perm/wash buffer (BD Biosciences, CA, USA). Cross-reactive flow cytometry human antibodies anti-CD3 APC-Cy7 (clone SP34-2), anti-CD8 PE-Cy7 (clone RPA-T8), anti-HLA-DR APC (clone G46-6), and anti-ki67 PE (B56) were purchased from BD Pharmingen (Franklin Lakes, NJ, USA). Anti-CD4 PerCP-Cy5.5 (clone OKT4) was obtained from Biolegend (San Diego, CA, USA). Flow cytometry acquisition was performed on a BD FACSVerseTM flow cytometer (BD, Franklin Lakes, NJ, USA) and flow cytometric data analysis was performed using FlowJo vX.0.7 (TreeStar, Ashland, OR, USA).
Zhang M.X., Song T.Z., Zheng H.Y., Wang X.H., Lu Y., Zhang H.D., Li T., Pang W, & Zheng Y.T. (2019). Superior intestinal integrity and limited microbial translocation are associated with lower immune activation in SIVmac239-infected northern pig-tailed macaques (Macaca leonina). Zoological Research, 40(6), 522-531.
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3

Comprehensive Phenotypic Analysis of CD8+ T Cells

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Flow cytometric analysis was performed using the following antibodies: anti-glut1 PE (202915) from R&D Systems, anti-KLRG1 PE (MAFA) from Miltenyi Biotec, anti-CD8 PerCP (SK1), anti-CD45RA BV605 (HI100), anti-CD45RA APC (HI1000), anti-CD27 BV421 (O323), anti-CD27 FITC (O323), anti-CD28 BV785 (CD28.2), and anti-CCR7 PECy7 (G043H7) from BioLegend. anti-Ki67 PE (B56; BD Bioscience), anti-p-p53 AF674 (Ser15, 16G8; Cell Signaling) and anti-pAMPK (T172, 40H9; Cell Signaling) were used for intracellular staining using solution AB (ThermoFisher). All samples were analysed using a LSR Fortessa (BD Biosciences) and the resulting data examined using FlowJo software (BD Bioscience). UMAP analysis was performed using FlowJo on down sampled CD8+ T cell populations (5,000 events), with standard parameters (nearest neighbours = 15, minimum distance = 0.5) and clustered via flowSOM.
Magnetic beads were used to isolate CD8+ T cells by positive selection according to the manufacturer’s instructions (Miltenyi Biotec). The purity of T cell subsets was assessed by flow cytometry.
Callender L.A., Carroll E.C., Garrod-Ketchley C., Schroth J., Bystrom J., Berryman V., Pattrick M., Campbell-Richards D., Hood G.A., Hitman G.A., Finer S, & Henson S.M. (2021). Altered Nutrient Uptake Causes Mitochondrial Dysfunction in Senescent CD8+ EMRA T Cells During Type 2 Diabetes. Frontiers in Aging, 2, 681428.
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4

Multiparametric Flow Cytometry Profiling

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Human mAbs that cross-react with rhesus macaques were used at their optimal concentrations in this study. Anti-CD38-FITC (clone AT-1) was obtained from StemCell Technologies. Anti-PD-1-PE (eBioJ105) and anti-CD27-PE-Cy5 (O323) were obtained from eBioscience. Anti-CCR5-PE (3A9), anti-CD14-APC (M5E2), anti-CD28-APC (CD28.2), anti-CD20-APC-H7/FITC (2H7), anti-CD3-APC-Cy7 (SP34-2), anti-CD4-PerCP-Cy5.5 (L200), anti-CD8-PE-Cy7 (RPA-T8), anti-CD80-FITC (L307.4), anti-CD86-PE (FUN-1), anti-CD95-FITC/PE-Cy7 (DX2), anti-CXCR4-PE-Cy7 (12G5), anti-HLA-DR-APC (G46-6) and anti-Ki67-PE (B56) were all obtained from BD Biosciences. Polyclonal anti-IgD-biotin was obtained from Southern Biotech, and streptavidin-PE-Cy7 was obtained from BioLegend.
Zheng H.Y., Zhang M.X., Chen M., Jiang J., Song J.H., Lian X.D., Tian R.R., Zhang X.L., Zhang L.T., Pang W., Zhang G.H, & Zheng Y.T. (2017). Accelerated disease progression and robust innate host response in aged SIVmac239-infected Chinese rhesus macaques is associated with enhanced immunosenescence. Scientific Reports, 7, 37.
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