A volume of 5 mL semen was diluted with 45 mL of triladyl solution (Minitüb GmbH, Tiefenbach, Germany) and mixed on a ThermoMixer (Eppendorf, Hamburg, Germany) with a mixing frequency of 300 rpm at 34.0 °C. After 30 min of mixing, the final concentration of sperm was adjusted to 80 × 106 sperm/mL, using a Makler counting chamber (Sefi-Medical Instruments, Haifa, Israel). The motility and progressiveness of sperm were analyzed using a microscope equipped with a computer-assisted sperm analyzer (CASA; Proiser, Paterna, Spain) with at least 200 sperm per bull. To analyze the sperm morphology, more than 200 sperm per bull were smeared on a glass slide (Paul Marienfeld GmbH & Co.KG, Lauda-Königshofen, Germany) and stained, using the Differential Quik III stain kit (Polysciences, Warrington, PA, USA). The stained slide was allowed to air dry and observed with a bright field microscope (Olympus, Tokyo, Japan) according to the criteria of sperm morphology [38 (link)].
Differential quik 3 stain kit
Manufactured by Polysciences
Sourced in United States
The Differential Quik III Stain Kit is a laboratory product used for staining microscopic biological samples. It is designed to provide a quick, reliable, and consistent way to differentiate and visualize various cellular components within the sample.
Automatically generated - may contain errors
Lab products found in correlation
Propidium iodide, by BD (2 mentions)
Vero cell, by American Type Culture Collection (2 mentions)
Thermomixer, by Eppendorf (1 mentions)
Bright field microscope, by Olympus (1 mentions)
Glass slide, by Marienfeld (1 mentions)
Atovaquone, by Merck Group (1 mentions)
Dihydroartemisinin dha, by Merck Group (1 mentions)
Sytox green, by BD (1 mentions)
Chloroquine cq, by Merck Group (1 mentions)
Atcc pra 405d, by American Type Culture Collection (1 mentions)
3 protocols using differential quik 3 stain kit
1
Bovine Sperm Characterization Protocol
2
Antimalarial Drug Evaluation Protocol
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Antimalarial drugs, including CQ and atovaquone, were used as control and acquired from Sigma-Aldrich (St. Louis, Missouri, USA). Propidium iodide and SYTOX Green solution were purchased from BD Pharmingen (San Diego, California, USA) and Life Technologies (Grand Island, New York, USA), respectively. The Differential Quik III Stain Kit was purchased from Polysciences, Inc. (Valley Road, Warrington, Pennsylvania, USA).
CQ-sensitive strains (P. falciparum 3D7 (American Type Culture Collection, ATCC PRA-405D) and P. falciparum D6 (ATCC MRA-285)) and Vero cells were purchased from ATCC (Manassas, Virginia, USA).
CQ-sensitive strains (P. falciparum 3D7 (American Type Culture Collection, ATCC PRA-405D) and P. falciparum D6 (ATCC MRA-285)) and Vero cells were purchased from ATCC (Manassas, Virginia, USA).
3
Antimalarial Combination Therapy Evaluation
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Chloroquine (CQ), Atovaquine, and Dihydroartemisinin (DHA) were from Sigma-Aldrich (St. Louis, MO, USA). The Differential Quik III Stain Kit was obtained from Polysciences Inc. (Valley Road Warrington, PA, USA). Propidium iodide (PI) and SYTOX Green solution were brought from BD Pharmingen, Inc. (San Diego, CA, USA) and Life Technologies (Grand Island, NY, USA), respectively. The CQ-sensitive strain Plasmodium falciparum (3D7) (American Type Culture Collection, ATCC PRA-405D) and Vero cells were obtained from American Type Culture Collection (ATCC) (Manassas, VA, USA). The Atovaquone-resistant rodent malaria strain Plasmodium berghei NAT (MRA-415), was obtained from BEI Resources (Manassas, VA, USA).
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