Masslynx ver 4

We used a Xevo TQ-S mass spectrometer equipped with Acquity I Class UPLC (Waters Corporation, Milford, MA). CORTECS C18 column (2.7 µm particle size, 4.6 mm $\times$ 100 mm i.d.) (Waters Corporation, #186007377) for the separation of fatty acids and their derivatives. LC conditions are as follow; 45% of solvent A (H₂O + 0.01% acetic acid) and 55% of solvent B (MeOH + 0.01% acetic acid) with a flow rate of 0.6 mL/min was initially set at 0 min., then gradient was set for 85% B at 10 min, 98% B at 18 min, 100% B at 20 min, back to 55% B at 30 min. The capillary voltage was −2.5 kV, desolvation temperature was 600 °C, desolvation gas flow was 1100 L/h, cone gas was 150 L/h, and nebulizer pressure was 7.0 bar with a source temperature of 150 °C. MassLynx ver. 4.1 software (Waters Corporation) was used for the operation and recording of the data. Data were expressed as the change in the relative abundance of pro-homeostatic lipid mediators normalized with the internal standards.