B6 mice

Manufactured by Jackson ImmunoResearch

Sourced in United States, Montenegro

B6 mice are a common laboratory mouse strain, also known as C57BL/6. They are a widely used model in biomedical research due to their genetic stability and well-characterized physiology.

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57 protocols using b6 mice

Biodistribution of EVs in Mice

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B6 mice (Jackson laboratory) (10 weeks old males) were used in the study. Animals were kept in specific pathogen-free facilities with regular diet and regular light/dark cycles, and regular ambient temperature and humidity in the Animal Resources Center at Northwestern University. All animal procedures were complied with the NIH Guidelines for the Care and Use of Laboratory Animals and were approved by the respective Institutional Animal Care and Use Committees. EVs isolated from HEK-ACE2 cells (as described above), were stained with PKH67 (Sigma, PKH67GL-1KT) and PKH67 dilutant control prepared similarly without the presence of EVs. 6–9 weeks old female and male B6 mice (Jackson Laboratory) were deeply anesthetized using isoflurane, and the EV suspension in 25 µL was pipetted into the right nostril of animals. After 24 h, animals were sacrificed, and lungs, brain, heart, liver, kidney, and spleen were isolated, rinsed with PBS. The organs were then imaged and total fluorescence efficiency was quantified using the IVIS imaging system ex vivo imaging was done using LAGO from Spectral Imaging Instruments and images were analyzed using AURA imaging software.

El-Shennawy L., Hoffmann A.D., Dashzeveg N.K., McAndrews K.M., Mehl P.J., Cornish D., Yu Z., Tokars V.L., Nicolaescu V., Tomatsidou A., Mao C., Felicelli C.J., Tsai C.F., Ostiguin C., Jia Y., Li L., Furlong K., Wysocki J., Luo X., Ruivo C.F., Batlle D., Hope T.J., Shen Y., Chae Y.K., Zhang H., LeBleu V.S., Shi T., Swaminathan S., Luo Y., Missiakas D., Randall G.C., Demonbreun A.R., Ison M.G., Kalluri R., Fang D, & Liu H. (2022). Circulating ACE2-expressing extracellular vesicles block broad strains of SARS-CoV-2. Nature Communications, 13, 405.

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Reverse Light Cycle in C57BL/6J Mice

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Subjects were adult (8–10 weeks old) male C57BL/6J (B6) mice, obtained either from the Jackson Laboratory (Sacramento, CA; cohorts 1–2) or the Psychological and Brain Sciences vivarium at UCSB (cohorts 3–5). The mice, bred in-house, were raised under a 12-h regular light cycle (lights on: 0700 h) until approximately 10 days prior to the onset of drinking procedures, at which time they were transferred to an adjacent colony room under a 12-h reverse light cycle (lights on: 2200 h). B6 mice from the Jackson Laboratory were allowed 10 days to acclimatize to the reverse-cycle housing conditions prior to the onset of drinking procedures. After the final day of drinking, mice were transferred back to the regular light cycle and allowed to re-acclimatize for 10 days prior to CPP testing. This was done to lower the spontaneous activity of the animals and augment the probability of detecting MA-induced locomotor hyperactivity. Mice were housed in groups of 4 on a ventilated rack and only separated from their cagemates during experimental procedures, which were all approved by the Institutional Animal Care and Use Committee of the University of California Santa Barbara and conducted in accordance with the Guide to the Care and Use of Laboratory Animals (2014).

Fultz E.K, & Szumlinski K.K. (2017). Prior binge-drinking history promotes the positive affective valence of methamphetamine in mice. Drug and alcohol dependence, 183, 150-154.

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Mice Strain Comparison Study

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Both male and female adult B6 mice purchased from The Jackson Laboratories were studied.

Zhou Y, & Kreek M.J. (2019). Combination of clinically utilized kappa opioid receptor agonist nalfurafine with low-dose naltrexone reduces excessive alcohol drinking in male and female mice. Alcoholism, clinical and experimental research, 43(6), 1077-1090.

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Acclimation of B6 Mouse Colony

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Adult male (PND 56 ± 3) B6 mice were purchased from the Jackson Laboratory (Bar Harbor, ME) and allowed 10-14 days to acclimate the colony room and single housing prior to experimentation. Animals were maintained on a 12-hour reverse light/dark cycle with lights OFF at 0700 with temperature and humidity held constant near 20° C and 50%, respectively. Food and water were available ad libitum. Principles of laboratory animal care were followed and experiments were performed under a protocol approved by the IUPUI School of Science Institutional Animal Care and Use Committee.

Fritz B.M, & Boehm SL I.I. (2015). Adenosinergic regulation of binge-like ethanol drinking and associated locomotor effects in male C57BL/6J mice. Pharmacology, biochemistry, and behavior, 135, 83-89.

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Animal Study Protocol Approval

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Twelve-week-old male B6 mice were obtained from the Jackson Laboratory (Bar Harbor, ME) and housed in Loma Linda University Animal Care Facility. All in vivo animal study protocols were reviewed and approved by both the Institutional Animal Care and Use Committee of Loma Linda University and the Animal Care and Use Review Office of the U.S. Army Medical Research and Materiel Command (USAMRMC) of the U.S. Department of Defense.

Wasnik S., Lakhan R., Baylink D.J., Rundle C.H., Xu Y., Zhang J., Qin X., Lau K.H., Carreon E.E, & Tang X. (2019). Cyclooxygenase 2 augments osteoblastic but suppresses chondrocytic differentiation of CD90+ skeletal stem cells in fracture sites. Science Advances, 5(7), eaaw2108.

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Mouse Feeding and Drink Preference

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Thirty‐one male (M) and 22 female (F) B6 mice were purchased from Jackson Laboratories, approximately 6 weeks of age, housed individually, and, upon their arrival, were placed in a 12‐hr:12‐hr LD cycle with RC. Male and female mice were placed into either a food preference experiment (HFD and RC) or a drink preference experiment (10% EtOH and H₂O). Two control groups of males (N = 6) and females (N = 5) consuming only RC and H₂O were used to assess overall fluid and food consumption and activity profiles between sexes.

Gelineau R.R., Arruda N.L., Hicks J.A., Monteiro De Pina I., Hatzidis A, & Seggio J.A. (2017). The behavioral and physiological effects of high‐fat diet and alcohol consumption: Sex differences in C57BL6/J mice. Brain and Behavior, 7(6), e00708.

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OT-I Mouse T Cell Protocol

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Seven-week-old B6 mice were purchased from The Jackson Laboratories (Bar Harbor, ME). T cell receptor (TCR) transgenic OT-I mice (Falk et al.,1993) were bred onto a Thy1.1 background and maintained as a breeding colony at the AAALAC-approved Animal Resources Center at the University of Texas Medical Branch (UTMB). All animal work was approved by the Institutional Animal Care and Use Committees of with oversight of staff veterinarians.

Winkelmann E.R., Widman D.G., Xia J., Johnson A.J., van Rooijen N., Mason P.W., Bourne N, & Milligan G.N. (2014). Subcapsular sinus macrophages limit dissemination of West Nile virus particles after inoculation but are not essential for the development of West Nile virus-specific T cell responses. Virology, 0, 278-289.

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Alcohol Preference in Male and Female Mice

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For all DID studies, male B6 mice were purchased from The Jackson Laboratory (Bar Harbor, ME). Mice were delivered at 7 weeks of age and were allowed two weeks to acclimate to the reverse light cycle before testing started. For all two-bottle choice studies, HAP1 male and female animals from the 47^th, 51^st and 53^rd generations were obtained from Dr. Nicholas Grahame at IUPUI. All animals were single housed 7 days prior to onset of testing, and between 9 and 10 weeks of age at onset of testing. Lights were maintained on a 12-hr reverse light-dark schedule with lights out at 8, 9, or 11am. Animals had access to food and water at all times, apart from during DID ethanol presentation when water was not available. All procedures were approved by the IUPUI School of Science Institutional Animal Care and Use Committee and conformed to the Guide for the Care and Use of Laboratory Animals (The National Academic Press, 2003).

Kasten C.R., Blasingame S.N, & Boehm SL I.I. (2014). Bidirectional enantioselective effects of the GABAB receptor agonist baclofen in two mouse models of excessive ethanol consumption. Alcohol (Fayetteville, N.Y.), 49(1), 37-46.

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Pristane-Induced Lung Injury Model

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B6 mice (Jackson) maintained under specific pathogen free conditions were injected with pristane (Sigma-Aldrich, 0.5 ml i.p.), mineral oil (MO; C.B. Fleet Co.), PBS, or left untreated. PEC were collected 14 days later. Some mice were treated with pristane on d0 plus either LXR agonist T0901317 (200 µg in DMSO per mouse i.p. daily) or DMSO alone. Mice received T0901317 on d1–d14 or on d1–d3, d3–d14, or d7–d14 only. On d14, lungs were evaluated for DAH by gross inspection of the excised lungs followed by microscopic confirmation as described previously (3 (link)). This study was carried out in accordance with the recommendations of the Animal Welfare Act and US Government Principles for the Utilization and Care of Vertebrate Animals and was approved by the UF IACUC.

Han S., Zhuang H., Shumyak S., Wu J., Xie C., Li H., Yang L.J, & Reeves W.H. (2018). Liver X Receptor Agonist Therapy Prevents Diffuse Alveolar Hemorrhage in Murine Lupus by Repolarizing Macrophages. Frontiers in Immunology, 9, 135.

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Genetic Manipulation of Lipid Metabolism in Mice

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Animal experiments were conducted in accordance with Institutional Animal Care and Use Committee approved protocols at the La Jolla Institute for Allergy & Immunology (#AP126-NB3) and the University of California, San Diego (S16098). B6 mice were purchased from Jackson Laboratory (JAX#000664). Generation of Acp1 floxed (Acp1^fl) mice carrying Cre expressed under the adiponectin (Adipoq) promoter was described(Stanford, 2017 (link)). LMPTP KO mice were generated by crossing Acp1^fl mice with mice carrying Cre expressed under the cytomegalovirus promoter (JAX #006054), followed by crossing to B6 mice to remove the Cre transgene. To generate DIO mice, male littermate Acp1^fl/fl Cre⁺ and Cre^– littermate mice were fed high-fat diet (HFD) chow containing 60 kcal % fat (Research Diets) for 12 months starting at 4–8 weeks of age.

Stanford S.M., Collins M., Diaz M.A., Holmes Z.J., Gries P., Bliss M.R., Lodi A., Zhang V., Tiziani S, & Bottini N. (2021). The low molecular weight protein tyrosine phosphatase promotes adipogenesis and subcutaneous adipocyte hypertrophy. Journal of cellular physiology, 236(9), 6630-6642.

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