Lentivirus based linc00472 targeting shrna vectors

HK-2 cells and 769-P cells were purchased from National Collection of Authenticated Cell Cultures (Shanghai, China). HK-2 cells were grown in DMEM/F12 (1:1) Medium with 10% fetal bovine serum (Gibco, California, USA). 769-P cells were cultured in RPMI 1640 Medium with 10% fetal bovine serum. All medium were supplemented with 10 U/ml of penicillin-streptomycin and all cells were cultured in a 5% CO2 humidified incubator at 37 °C. The control shRNA and Lentivirus-based LINC00472-targeting shRNA vectors were purchased from GENECHEM (Shanghai, China). The sequence of shLINC00472#1 is “GCAACAGAAGTATGTGCAA”, shLINC00472#2 is “GCCAGTATATACTGAACAT”. HK-2 cells and 769-P cells were transiently transfected with these vectors and screened by puromycin at a concentration of 2 μg/ml to generate stable monoclonal cell lines.

U251 cells, A172 cells, and Jurkat cells were purchased from the National Collection of Authenticated Cell Cultures. U251 and A172 were grown in DMEM medium with 10% fetal bovine serum (Gibco, California, USA). Jurkat cells were cultured in RPMI 1640 Medium with 10% fetal bovine serum. All medium was supplemented with 10 U/ml of penicillin-streptomycin, and all cells were cultured in a 5% CO2 humidified incubator at 37°C. The control shRNA and Lentivirus-based LINC00472-targeting shRNA vectors were purchased from GENECHEM (Shanghai, China). U251 cells were transiently transfected with these vectors and screened by puromycin at a 2 mg/ml concentration to generate stable monoclonal cell lines.