Innotest immunoassay

Manufactured by Fujirebio

Sourced in Belgium

The Innotest® immunoassay is a laboratory equipment product developed by Fujirebio. It is used for the detection and quantification of specific analytes in biological samples through immunological methods.

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Lab products found in correlation

Simoa kit, by Quanterix (1 mentions) Meso scale discovery (msd), by Mesoscale (1 mentions) Lumipulse, by Fujirebio (1 mentions) Nf light assay, by UmanDiagnostics (1 mentions) Cobas e601 analyzer, by Roche (1 mentions) Lumipulse g1200, by Fujirebio (1 mentions) Analyzer 1 2p, by EUROIMMUN (1 mentions)

4 protocols using innotest immunoassay

Alzheimer's CSF Biomarker Analysis Protocol

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Lumbar CSF sampling and analysis were conducted according to the Alzheimer’s Association Flow Chart.^{37 (link)} As previously described, the analysis of P-tau217 assays was performed using the Meso Scale Discovery (MSD) platform.^{38 (link)} Total tau (T-tau) was quantified using Innotest® immunoassay (Fujirebio; Gent, Belgium). CSF Aβ42 and Aβ40 levels were measured using MSD or Fujirebio Lumipulse assays according to the manufacturer’s instructions, and the ratio values were subsequently calculated. NfL was measured using a Simoa kit (Quanterix; Billerica, MA). NPTX2 was measured in the facilities of ADx (Gent, Belgium) using a research grade sandwich ELISA. The number of available data per group for each of the CSF and imaging biomarkers together with the corresponding mean ± SD values are provided in Supplementary Table 3.

Ahmadi K., Pereira J.B., Berron D., Vogel J., Ingala S., Strandberg O.T., Janelidze S., Barkhof F., Pfeuffer J., Knutsson L., van Westen D., Palmqvist S., Mutsaerts H.J, & Hansson O. (2022). Gray matter hypoperfusion is a late pathological event in the course of Alzheimer’s disease. Journal of Cerebral Blood Flow & Metabolism, 43(4), 565-580.

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CSF Biomarkers for Alzheimer's Diagnosis

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CSF Aβ42 and CSF p-tau181 was quantified using Innotest immunoassay (Fujirebio). Aβ positivity was defined using a cutoff of <783.7 pg/mL for CSF Aβ42 and tau positivity using a cutoff of >58.8 pg/mL for CSF p-tau181. Both cutoffs were derived using mixture modeling¹⁸ based on CSF levels from all participants who had undergone lumbar puncture at baseline in the BioFINDER-2 cohort by October 2019 (n = 949).

Tideman P., Stomrud E., Leuzy A., Mattsson-Carlgren N., Palmqvist S, & Hansson O. (2022). Association of β-Amyloid Accumulation With Executive Function in Adults With Unimpaired Cognition. Neurology, 98(15), e1525-e1533.

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CSF Biomarker Quantification Protocol

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CSF was obtained by lumbar puncture (LP), in the morning, using a 22G Sprotte needle.
LP was performed at level L3/L4 or L4/L5 and CSF (at least 4 ml) was collected in polypropylene tubes. Samples were centrifuged at 2000xg during 10 minutes. Supernatants were stored in polypropylene cryovials at -80°C until analysis. Amyloid-beta 1-42 (Aβ 1-42 ), total-tau (t-tau), and phospho-tau (p-tau) were quantified using INNOTEST immunoassays from Fujirebio (Belgium) 19 , following instructions from the manufacturer.
NfL quantification was performed using the NF-Light Assay from Uman Diagnostics (Sweden) according to manufacturer's instructions 20 .

Niikado M., Chrem-Méndez P., Itzcovich T., Barbieri-Kennedy M., Calandri I., Martinetto H., Serra M., Calvar J., Campos J., Russo M.J., Pertierra L., Allegri R., Sevlever G, & Surace E.I. (2019). Evaluation of Cerebrospinal Fluid Neurofilament Light Chain as a Routine Biomarker in a Memory Clinic. The journals of gerontology. Series A, Biological sciences and medical sciences, 74(4).

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Comparative Analysis of CSF Biomarkers

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CSF concentrations of Aβ42, Aβ40, total Tau, and p-Tau 181 were measured with commercially available immunoassays, using the manufacturer's procedures. Four different methods were used: (1) The Elecsys immunoassays using the cobas e601 analyzer (Roche Diagnostics GmbH). (2) The INNOTEST immunoassays (Fujirebio Europe, Gent, Belgium). (3) The Lumipulse G1200 (Fujirebio Europe, Gent, Belgium). (4) The Euroimmun analyzer I-2P (Euroimmun AG, Luebeck, Germany).
Some centers (Paris, Montpellier, and Lille) contributed 2 patient cohorts because they used 2 different methods over time for the dosage of biomarkers. CSF samples in the ADNI study were analyzed using Elecsys immunoassays. We decided not to include older CSF ADNI data from the Luminex platform because of the long delay, approximately 5 years, between the CSF and tau PET measures. More complete information regarding CSF data in the ADNI is available online.²⁹

Dumurgier J., Sabia S., Zetterberg H., Teunissen C.E., Hanseeuw B., Orellana A., Schraen S., Gabelle A., Boada M., Lebouvier T., Willemse E.A., Cognat E., Ruiz A., Hourregue C., Lilamand M., Bouaziz-Amar E., Laplanche J.L., Lehmann S., Pasquier F., Scheltens P., Blennow K., Singh-Manoux A, & Paquet C. (2022). A Pragmatic, Data-Driven Method to Determine Cutoffs for CSF Biomarkers of Alzheimer Disease Based on Validation Against PET Imaging. Neurology, 99(7), e669-e678.

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