HCT8 and RKO cells (2 × 105 cells per well) were seeded into 6‐well plates. HCT8 cells were treated with 3‐bp (25 μM) and/or oxaliplatin (25 μM), and RKO cells were treated with 3‐bp (25 μM) and/or oxaliplatin (25 μM) for 48 h. A total of 1 × 106 cells per sample were stained with Cell Cycle Staining Kits (Multi Sciences, CCS012) and detected by CytoFLEX (Beckman Coulter). The data were plotted, and a curve was fitted using ModFit LT software (PO Box 247).
Cell cycle staining kit
Manufactured by MultiSciences Biotech
Sourced in China, United States
The Cell Cycle Staining Kit is a laboratory tool used to analyze the cell cycle stage of a sample population. The kit contains the necessary reagents to stain cellular DNA, allowing for the identification and quantification of cells in different phases of the cell cycle through flow cytometry or other analytical methods.
Automatically generated - may contain errors
Lab products found in correlation
Annexin 5 fitc pi apoptosis kit, by MultiSciences Biotech (16 mentions)
Cytoflex, by Beckman Coulter (10 mentions)
Annexin 5 apc 7 aad apoptosis kit, by MultiSciences Biotech (10 mentions)
Facscalibur flow cytometer, by BD (9 mentions)
Cytoflex s, by Beckman Coulter (7 mentions)
Facscalibur, by BD (7 mentions)
Flow cytometry, by Beckman Coulter (6 mentions)
Flow cytometry, by BD (5 mentions)
Annexin 5 fitc pi apoptosis detection kit, by MultiSciences Biotech (4 mentions)
Software, by FlowJo (4 mentions)
Modfit lt software, by Verity Software House (4 mentions)
Modfit lt 3, by Verity Software House (4 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (4 mentions)
Annexin 5 fitc pi apoptosis assay kit, by MultiSciences Biotech (3 mentions)
Z vad fmk, by Selleck Chemicals (3 mentions)
Cleaved caspase 3, by Cell Signaling Technology (3 mentions)
Annexin 5 fitc apoptosis detection kit, by Keygen Biotech (3 mentions)
Annexin 5 fitc pi apoptosis detection kit, by BD (3 mentions)
Gallios flow cytometry, by Beckman Coulter (3 mentions)
Lsrii flow cytometer system, by BD (3 mentions)
215 protocols using cell cycle staining kit
1
Cell Cycle Analysis of Colorectal Cancer Cells
2
Cell Cycle Analysis by Flow Cytometry
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Flow cytometry and Cell Cycle staining kits (Multisciences, Hangzhou, China) were utilized to analyse the cell cycle. Referring to the manufacturer's protocol, the cells were digested into a single cell suspension with 0.25% trypsin (without EDTA), and approximately 6 × 105 cells were collected by centrifugation at 1000 RPM. After washing twice with PBS, 1 ml DNA staining solution and 10 μl permeabilization solution were added. Immediately after staining at room temperature and avoiding light for 30 min, a FACS Calibur Flow Cytometer (BD Biosciences, USA) was used for detection. The data were quantified using Modfit LT 5.0 software.
3
BRCA Cell Cycle Evaluation
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BRCA cells were harvested from a six‐well plate and fixed with 75% alcohol at −20°C for at least 24 h. The staining steps were performed following the Cell Cycle Staining Kit protocol (MULTI SCIENCES). After hydrating the cells, 1 mL of DNA staining solution was added to each sample and samples were incubated at 20°C for 30 min. The cell cycle data were collected by Cytoflex (Beckman).
4
Cell Cycle and Apoptosis Analysis of Transfected GBM Cells
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Transfected GBM cells in logarithmic growth were collected and processed with the Cell Cycle Staining Kit (Multi Sciences, Hangzhou, China) for cell cycle analysis. After washing with PBS, cells were fixed with 70% ice-cold ethanol, incubated with Cell Cycle Staining Kit for 30 min in the dark, and analyzed by flow cytometry. In other experiments, treated cells in logarithmic growth were harvested and stained with the Annexin V-FITC Apoptosis Detection Kit (Multi Sciences). After washing with PBS and incubating with Annexin V/propidium iodide for 30 min in the dark, cells were analyzed by flow cytometry.
5
Evaluating MEC Apoptosis and Cell Cycle
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MEC apoptotic rate was evaluated using the Annexin V-FITC/PI apoptosis kit (7Sea Biotech, Shanghai, China) and MEC cell cycle was measured by Cell cycle staining Kit (MultiSciences, Hangzhou, China) according to the manufacturer’s instructions.
6
Cell Cycle Analysis of Lipid Signaling
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Cells were seeded at 50% confluence in six-well plates overnight and then treated with fresh 10% medium containing PF-543 (15 μM), S1P (5 μM) or TY-52156 (5 μM) for 48 h. Cell cycle analysis was performed with cell cycle staining kit (Multi Sciences, CCS012, Hangzhou, China) according to the manufacturer’s instructions. Finally, flow cytometry analysis of cells was performed on a BD FACS Aria II flow cytometer (BD Biosciences, LSR Fortessa, Franklin, NJ, USA).
7
Cell Cycle Analysis of Curcumin, Irinotecan, and SICN
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Cycle in cells was visualized using a MultiSciences Cell Cycle Staining Kit. HGC-27 cells were placed in a 12-well plate at a density of 1 × 105 cells per well and further incubated for 24 h and treated with curcumin, irinotecan hydrochloride, or SICN nanoparticles at 3.125 μM/L concentration for 48 h. Negative control was prepared by incubating cells in a medium without any drugs. Cells were harvested, washed twice with cold PBS, and stained with MultiSciences Cell Cycle agents according to the manufacturer’s instructions. Stained cells were analyzed by a BD FACSVerse flow cytometer.
8
Apoptosis and Cell Cycle Analysis of PDAC
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The apoptosis of PDAC cells was examined using an Annexin V-FITC/PI apoptosis kit (70-AP101-100; MultiSciences, China). PDAC cells were collected (2 × 105 per well) and stained with Annexin V-FITC/PI. The positive control group was treated by heat shock (55°C, 10 min) and stained with Annexin V-FITC/PI. Then, the percentage of apoptotic cells was evaluated by flow cytometry in a Becton-Dickinson FACScan System (Franklin Lakes, USA). Cell cycle was analysed by Cell cycle staining Kit (CCS012; MultiSciences). PDAC cells were collected (2 × 106 per well) and stained with PI. Then, the cell cycle distribution was evaluated by flow cytometry.
9
Cell Cycle Analysis by Flow Cytometry
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First, cells were serum-starved to synchronize the cell cycle. Cells were then collected, washed in phosphate-buffered saline (PBS) and fixed in 70% ethanol at −20 °C for 24 h. After fixing, cells were rehydrated with pre-cooled PBS, stained with a DNA staining solution (Cell Cycle Staining Kit, Multi Sciences, Hangzhou, China), then incubated for 30 min, and finally tested using a FACSCalibur Flow Cytometer (BD Biosciences, San Jose, CA, USA). Cell cycle profiles were analyzed by Modifit software (BD Biosciences).
10
Cell Cycle Analysis by Flow Cytometry
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Cells were treated with the Cell Cycle Staining Kit (MultiSciences Biotech Ltd., China) after washing with cold phosphate-buffered saline. Flow cytometry (Beckman, USA) was used to detect the percentage of cells existing at different cell cycle phases.
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