Rosa26 stop eyfp

The ROSA26-eGFP-DTA (ref. 43 (link)), the ROSA26-stop-EYFP (ref. 44 (link)), the Rag1 knockout^{14 (link)} (Rag1^−/−) and the 2D2 transgenic^{12 (link)} mice were purchased from the Jackson Laboratory. The ROSA26-eGFP-DTA and the ROSA26-stop-YFP mice were bred to the Plp1-CreER^T transgenic mice^{9 (link)} to generate the Plp1-CreER^T;ROSA26-eGFP-DTA (DTA) and Plp1-CreER^T;ROSA26-stop-EYFP (reporter) mice, as previously described^{8 (link)}. All mouse studies were conducted in compliance with The University of Chicago’s and Northwestern University’s Animal Care and Use Committee (IACUC) guidelines.

The following mice on a C57BL/6 background were purchased from The Jackson Laboratory and used at 10 weeks of age unless otherwise stated: Cxcl12^DsRed knockin (stock no. 022458) (23 (link)), which were used in isolation or crossed with the following: Tcrα^-/- (stock no. 002116, (24 (link)), Rag2^-/-(stock no. 008449) (25 (link)), Foxn1^Cre (stock no. 018448)(26 (link)) and Rosa26-stop-EYFP (stock no. 006148) (27 (link)). Control mice for experiments involving and Tcra^-/- and Rag2^-/- mice were heterozygous littermate controls. RANK^Venus BAC transgenic mice were generated as described previously (28 (link)). Husbandry, housing, and experimental methods involving mice were performed at the Biomedical Services Unit at the University of Birmingham in accordance with the local Ethical Review Panel and UK Home Office Regulations (Animal project Licence number P3ACFED06).