Span 80

Manufactured by Fujifilm

Sourced in Japan

Span 80 is a non-ionic surfactant used in various laboratory applications. It serves as a wetting agent, emulsifier, and solubilizer. The core function of Span 80 is to reduce surface tension and facilitate the dispersion of materials in aqueous or non-aqueous solutions.

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Lab products found in correlation

6 protocols using span 80

Preparation of W/O Emulsions with Tunable Compositions

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Cyclohexane, n-pentane, n-hexane, n-nonane, toluene, and sorbitan monooleate (Span 80, as an emulsifier) all were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan) and were used without further purification.
The preparation procedures for a W/O emulsion were as follows. First, solutions for an oil phase were prepared; the ratios of cyclohexane and one of the n-alkanes were adjusted to 100:0, 99.7:0.3, 99:1, and 90:10 (v:v), respectively. Then, toluene was added, and the concentration was adjusted to 5 g/L (i.e., 0.125 g of toluene per 25 mL of the solution for an oil phase). Also, 0.258 g of Span 80 was added to another vial container, and 25 mL of the solution for an oil phase were added. While stirring the solution with a homogenizer (4000 rpm), 0.773 mL of distilled water was added using a micropipette. The ratio between distilled water and the solution for the oil phase was 3:97 (v:v). The stirring was continued for 3 min, and a W/O emulsion was obtained. The final concentration of Span 80 was 10 g/L of a W/O emulsion.

Minami M., Nakata S, & Uchimura T. (2024). Sample preparation conditions for the real-time measurement of W/O emulsions by resonance-enhanced multiphoton ionization time-of-flight mass spectrometry. Analytical Sciences, 40(3), 573-577.

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Biomimetic Hydroxyapatite Microrods Synthesis

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Cyclohexane, CaCl₂, K₂HPO₄, and KOH were purchased from Wako Pure Chemical Industries and were of analytical grade. The three types of surfactants, namely, Span 80 (sorbitan monooleate), Tween 20 (polyoxyethylene (20) sorbitan monolaurate), and Tween 80 (polyoxyethylene (20) sorbitan monooleate) (a hydrophilic surfactant, HLB value: 15) [25] , were reagent grade products of Wako Pure Chemical Industries. Commercially available HA (Apatite HAP, monoclinic, Wako Pure Chemical Industries) was used as the HA microrods, for comparison. KBr for infrared (IR) analysis was purchased from Wako Pure Chemical Industries. Four proteins, namely, bovine serum albumin (A3059), bovine γ-globulin (G5009), equine skeletal muscle myoglobin (M0630), and chicken egg white lysozyme (L6876), were obtained from Sigma-Aldrich. Poly(l-lactic acid) (PLLA) was a gift from Toyota Motor Corp. The PLLA properties included the weight average molecular weight: 1.22×10⁵ (M_w/M_n = 3.0), optical purity: 98.5%, melting point: 174.0 °C, and glass transition temperature: 59.7 °C. Analytical grade 1,4-dioxane was purchased from Wako Pure Chemical Industries. All chemicals were used without further purification.

Tanaka T., Takai Y., Nagase A., Teraguchi K., Minbu H., Ochiai A., Kimura I, & Taniguchi M. (2019). Protein adsorption characteristics of nanoparticle-assembled hollow microspheres of hydroxyapatite and their composites with PLLA microporous membranes. Heliyon, 5(4), e01490.

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Scaffold DNA Self-Assembly Synthesis

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All staple strands of ssDNA were purchased from Eurofins Genomics Tokyo (Tokyo, Japan). Single-stranded M13mp18 viral DNA (as scaffold ssDNA) was purchased from Tilibit Nanosystems (Garching, Germany). The nonionic surfactant Span80 (Sorbitan, mono-(9Z)-9-octadecenoate) and liquid paraffin were purchased from Wako Pure Chemical Industries, Ltd. (Tokyo, Japan). All chemicals were used without further purification.

Watanabe T., Sato Y., Otaka H., Kawamata I., Murata S, & Nomura S.I. (2019). DNA Origami “Quick” Refolding inside of a Micron-Sized Compartment. Molecules, 25(1), 8.

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Droplet Flow Reactor for RNA Experiments

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The construction of the droplet flow reactor has been previously reported in detail^{34 (link),35 (link)}. Briefly, this system is composed of two tanks (505 μL), the inside solutions of which were mixed by magnetic stirrers (Fig. 1). The tanks were attached to electromagnetic coils to control the stirring intensity. The oil phase including mineral oil (Sigma) and surfactants (2% Span 80 (v/v, Wako) and 3% Tween 80 (v/v, Wako)) and the aqueous phase including the E.coli translation system were supplied into the first tank with two syringe pumps. The oil phase was used after saturation with some solutes as described previously¹⁷. In the first tank, droplets were prepared at 2 °C and supplied to the second tank. In the second tank, droplets contain the genomic and parasitic RNAs and fusions and divisions among the droplets were induced by mixing. Droplets in the second tank were removed at the same rate as the supply rate (dilution rate). The mixing intensities were controlled by changing the spinning period of the magnetic stirrers in the tanks. The details of the droplet flow reactor are shown in Fig. S6.

Yoshiyama T., Ichii T., Yomo T, & Ichihashi N. (2018). Automated in vitro evolution of a translation-coupled RNA replication system in a droplet flow reactor. Scientific Reports, 8, 11867.

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Optimizing W/O/W Emulsion Stability

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We investigated the relationship between the HLB value of the surfactant added to the organic phase and the stability of the W/O/W emulsion droplets. The surfactants were SPAN 85 (sorbitan trioleate, HLB value: 1.8, monomer, Wako Pure Chemical Industries, Japan), SPAN 80 (sorbitan monooleate, HLB value: 4.3, monomer, Wako Pure Chemical Industries, Japan), and SY-Glyster CRS-75 (polyglycerol esters of fatty acids, HLB value: 3, polymer, Sakamoto Yakuhin Kogyo, Osaka, Japan). These three surfactants were used to prepare three different organic phases consisting of decane (Wako Pure Chemical Industries, Ltd., Japan)/1% surfactant. Additionally, to verify the status of droplet formation using a decane organic phase supplemented with both monomeric and polymeric surfactants, decane was supplemented with both 1% (w/w) SPAN 80 and 0.5% (w/w) SY-Glyster CRS-75. These organic phases, inner aqueous phases (DMEM, Life Technologies, USA), and outer aqueous phases (PBS/2% PVA (The Nippon Synthetic Chemical Industry Co., Ltd., Japan)) were filled into syringes and then introduced into the microchannel using syringe pumps. Droplet formation was observed using a high speed camera mounted on an upright microscope.

Hirama H, & Torii T. (2015). One-to-one encapsulation based on alternating droplet generation. Scientific Reports, 5, 15196.

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Polymer-Based Emulsion Synthesis and Characterization

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All reagents were used as received. Pentaerythritol tetra(succinimidyloxyglutaryl) polyoxyethylene (TN-PEG, Mn = 10 kDa) and pentaerythritol tetra(aminopropyl) polyoxyethylene (TA-PEG, Mn = 10 kDa) were purchased from NOF Corporation, Japan.
Dextran (DEX, Mw = 32 -45 kDa), Span 80, tridecane, hexane, and toluene were purchased from FUJIFILM Wako Pure Chemical Corporation, Japan. Solsperse 19000 was kindly gifted from Lubrizol Corporation, Japan. Dextran labeled with fluorescein isothiocyanate (FITC-DEX, Mw = 250 kDa and 500 kDa) and trimethoxy(octadecyl)silane were purchased from Sigma-Aldrich, Japan. Reactive Blue 160 was purchased from MP Biomedicals, LLC. Deionized water with a resistance of 3 MΩ•cm was obtained by passing through Elix UV system.

Watanabe T., Motohiro I, & Ono T. (2019). Microfluidic Formation of Hydrogel Microcapsules with a Single Aqueous Core by Spontaneous Cross-Linking in Aqueous Two-Phase System Droplets. Langmuir : the ACS journal of surfaces and colloids, 35(6).

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