For western blotting, the proteins were subjected to SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) (Immobilon-P, Merck, Tokyo, Japan) membranes using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, Tokyo, Japan). Blocking was performed for at least 1 h with 15 ml 5% skim milk in TBS containing 0.1% Tween 20. After washing with TBS and incubation for at least 2 h with mouse anti-Strep-tag antibody (1:10,000, QIAGEN, Tokyo, Japan), the blots were rewashed. Incubation with a secondary goat anti-mouse IgG-horseradish peroxidase (HRP) (1:10,000, MBL, Nagoya, Japan) was performed for at least 1 h. Immunoreactive bands were visualized using the Immobilon ECL Ultra Western HRP Substrate (Merck K. K., Tokyo, Japan) on the Versa-Doc 4000 MP (BioRad, Hercules, CA, USA).
Mouse anti streptag antibody
Manufactured by Qiagen
Sourced in Japan
The Mouse anti-Streptag antibody is a laboratory reagent used for the detection and purification of proteins that have been tagged with the Strep-tag II peptide sequence. This antibody specifically binds to the Strep-tag II peptide, allowing for the identification and isolation of Strep-tagged proteins from complex samples.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using mouse anti streptag antibody
1
Western Blotting Protein Analysis
2
Western Blot Protein Detection Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For western blotting, the proteins were subjected to SDS-PAGE and transferred to polyvinylidene uoride (PVDF) (Immobilon-P, Merck, Tokyo, Japan) membranes using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, Tokyo, Japan). Blocking was performed for at least 1 h with 15 ml 5% skim milk in TBS containing 0.1% Tween 20. After washing with TBS and incubation for at least 2 h with mouse anti-Streptag antibody (1:10000, QIAGEN, Tokyo, Japan), the blots were washed again. Incubation with a secondary goat anti-mouse IgG-horseradish peroxidase (HRP) (1:10000, MBL, Nagoya, Japan) was performed for at least 1 h. Immunoreactive bands were visualized using the Immobilon ECL Ultra Western HRP Substrate (Merck K. K., Tokyo, Japan) on the Versa-Doc 4000 MP (BioRad, Hercules, CA, USA).
3
Western Blotting with SDS-PAGE and PVDF Transfer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For western blotting, the proteins were subjected to SDS-PAGE and transferred to polyvinylidene uoride (PVDF) (Immobilon-P, Merck, Tokyo, Japan) membranes using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, Tokyo, Japan). Blocking was performed for at least 1 h with 15 ml 5% skim milk in TBS containing 0.1% Tween 20. After washing with TBS and incubation for at least 2 h with mouse anti-Streptag antibody (1:10000, QIAGEN, Tokyo, Japan), the blots were rewashed. Incubation with a secondary goat anti-mouse IgG-horseradish peroxidase (HRP) (1:10000, MBL, Nagoya, Japan) was performed for at least 1 h. Immunoreactive bands were visualized using the Immobilon ECL Ultra Western HRP Substrate (Merck K. K., Tokyo, Japan) on the Versa-Doc 4000 MP (BioRad, Hercules, CA, USA).
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