Poly(ethylene terephthalate) (PET) surgical meshes (PETNF203) were purchased from Textile Development Associates, Inc. (Brookfield, CT, USA). Avidin-coated microspheres (CP01N) were purchased from Bangs Laboratories, Inc. (Fishers, IN, USA), sulfosuccinimidyl-4-O-(4,4-dimethoxytrityl) butyrate (Sulfo-SDTB) was obtained from bioWORLD (Dublin, OH, USA). Biotin N-hydroxysuccinimide ester was purchased from AppliChem (Darmstadt, Germany). Amine-terminated poly(ethylene glycol) dimer functionalized with biotin (Amine-PEG2-Biotin) and the Alexa Fluor 647 Microscale Protein labeling kit were purchased from Thermo Fisher Scientific (Dreieich, Germany). All other chemicals were purchased from Sigma-Aldrich (Darmstadt, Germany). Chemical reagents and solvents used for the synthesis were of analytical grade and used as received. All synthesis experiments were carried out in air. Water was deionized with a Milli-Q Reference water purification system manufactured by Millipore Corporation (Darmstadt, Germany). All reactions involving the modification of PET were carried out in a Snycore®® Reactor R-24 (Büchi, Flawil, Switzerland). Scanning electron microscopy (SEM) was performed on a Quanta 250 FEG microscope using an accelerating voltage of 20 kV in high vacuum from FEI (Hillsboro, OR, USA). The micrographs were then analyzed with xT Microscope Control (4.1.4).
Milli q reference water purification system
Manufactured by Merck Group
Sourced in Germany, United States, France
The Milli-Q® Reference Water Purification System is a water purification device designed to produce ultrapure water for laboratory and research applications. It utilizes a multi-stage purification process to remove impurities and contaminants from the input water, resulting in water that meets the highest purity standards.
Automatically generated - may contain errors
Lab products found in correlation
Sodium chloride (nacl), by Merck Group (3 mentions)
Trifluoroacetic acid hplc grade tfa, by Biosolve (2 mentions)
Methanol, by Merck Group (2 mentions)
Ethylene glycol, by Merck Group (2 mentions)
Ammonium heptamolybdate, by Merck Group (2 mentions)
Seveneasy ph meter s20, by Mettler Toledo (2 mentions)
3 aminopropyltriethoxysilane, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Triethylamine, by Merck Group (2 mentions)
Tetraethyl orthosilicate, by Merck Group (2 mentions)
Cholesterol, by Avanti Polar Lipids (2 mentions)
Alexa fluor 647 microscale protein labeling kit, by Thermo Fisher Scientific (1 mentions)
Quanta 250 feg microscope, by Thermo Fisher Scientific (1 mentions)
Amine peg2 biotin, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Merck Group (1 mentions)
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Cefadroxil, by Merck Group (1 mentions)
Nitric acid, by Merck Group (1 mentions)
Polypropylene tube, by Greiner (1 mentions)
29 protocols using milli q reference water purification system
1
PET Mesh Biotinylation and Avidin Functionalization
2
Phenolic Acids and Flavonoids Quantification
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Acetonitrile (gradient grade), methanol (HPLC grade), ammonium acetate, formic acid (≥99.8%) and all phenolic acids and flavonoids (Figure 1 ) were purchased from Sigma-Aldrich (St. Louis, MI, USA). Deionised water was treated with a Milli-Q Reference Water Purification System (Millipore SAS, Molsheim, France).
3
Collagen-based Enzymatic Assay Protocol
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Water was purified to Milli-Q water grade using an in-house Milli-Q® Reference Water Purification System (Millipore). Collagen–fluorescein (FITC) conjugate type I from bovine tendon was purchased from BioVision Inc. (Milpitas, CA, USA) The EnzChek™ gelatinase/collagenase assay kit, containing DQTM gelatin from pig skin fluorescein conjugate, was obtained from InvitrogenTM by Thermo Fisher Scientific Inc. (Waltham, MA, USA) (lot 2,174,593 and 2,281,586). GibcoTM Dulbecco’s phosphate-buffered saline (no calcium, no magnesium, pH 7.0 to 7.3; DPBS (2.67 mM KCl, 1.47 mM KH2PO4, 137.93 mM NaCl, 8.06 mM Na2HPO4-7H2O)) was purchased from Thermo Fisher Scientific Inc. Meanwhile, 2-propanol HPLC grade was purchased from VWR International. Acetonitrile HPLC-R (ACN), formic acid 99%, ULC/MS–CC/SFC (FA) and trifluoroacetic acid HPLC grade (TFA) were obtained from Biosolve B.V. Difluoroacetic acid for LC-MS (LiChropur, ≥97.5% (GC); DFA) was purchased from Sigma-Aldrich.
4
Microdialysis Evaluation of Cefadroxil Pharmacokinetics
5
Elemental stock and reference material preparation
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A multi-element stock standard (10 mg kg−1, IV71-A) and single-element standards (1000 mg kg−1) for Na, Ca, P, S, K and Fe were purchased from Inorganic Ventures, Christiansburg, VA, USA. Certified reference material (CRM) ERM-CE278k (mussel tissue) was purchased from European Reference materials and SLRS-6 river water CRM was purchased from National Research Council Canada. Nitric acid (65%, analytical grade) and hydrogen peroxide (30%, analytical grade) were purchased from Merck and Univar (Ajax Finechem Pty Ltd) respectively. Type 1 water was distilled and deionised using a Millipore Milli Q Reference water purification system (18.0 mΩ resistivity). Polypropylene tubes (15 mL) were purchased from Greiner. Minisart Syringe Filters (0.45 µm, cellulose acetate) were purchased from Sartrious, Germany. An Ohaus dry heat block was used for heating samples.
6
Detailed Reagent Preparation Protocol
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Ultrapure water was obtained through purification using a Milli-Q® Reference Water Purification System (Millipore). Dulbecco's phosphate buffered saline (no calcium, no magnesium, pH 7.0–7.3; PBS) was purchased from Thermo Fisher Scientific Inc. Tris-HCl buffer (50 mM, pH 7.4) was prepared with Trizma® base in ultrapure water and the pH was as adjusted using 1.0 M HCl. Egg yolk from chicken (powder), Trizma® base (≥99.9 %), hydrochloric acid (ACS reagent, 37 %; HCl) and Triton™ X-100 (BioXtra) were obtained from Sigma-Aldrich. Hoechst 33342 (trihydrochloride, trihydrate - 10 mg/mL solution in water; Hoechst) nucleic acid stain was obtained from Invitrogen™ by Thermo Fisher Scientific Inc., Gibco™. For the neutralisation assays, varespladib (Sigma-Aldrich) was used as a PLA2-inhibitor. Acetonitrile HPLC-R (ACN) and trifluoroacetic acid HPLC grade (TFA) were obtained from Biosolve B.V.
7
Quantitative Bioanalytical Method Development
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Certified reference drugs (Supplementary Table S1) and corresponding deuterated internal standards were obtained from Cerilliant (Round Rock, TX, USA). Working standard solutions for six calibrators, each containing all 53 analytes, were prepared by dilution of the stock solutions with acetonitrile. Additional working solutions for high and low positive controls, a low cut-off (LCO) and internal standard were also prepared in acetonitrile.
Liquid chromatography--mass spectrometry grade acetonitrile was obtained from Caledon Laboratory Chemicals (Halton Hills, ON, Canada). Formic acid (MS grade) and ammonium formate were obtained from Sigma-Aldrich (St. Louis, MO, USA). High purity deionized water was delivered by a Millipore Milli-Q® Reference water purification system (Molsheim, France).
Liquid chromatography--mass spectrometry grade acetonitrile was obtained from Caledon Laboratory Chemicals (Halton Hills, ON, Canada). Formic acid (MS grade) and ammonium formate were obtained from Sigma-Aldrich (St. Louis, MO, USA). High purity deionized water was delivered by a Millipore Milli-Q® Reference water purification system (Molsheim, France).
8
Synthesis of Stereospecifically Labeled NADH
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All the chemicals were purchased with high purity (typically > 99%) from Sigma-Aldrich and Merck, and these were used without further purification, unless otherwise indicated. The 5-deazariboflavin was a gift from the G. Tollin Lab (University of Arizona). The stereospecifically labeled nicotinamide nucleotide [4R-2H1]-NADH (with the deuterium in the A face of the nicotinamide ring, NADD) was synthetized enzymatically using [2H8]-propanol/alcohol dehydrogenase following previously described protocols [65 (link)]. Milli-Q water was obtained from a Milli-Q® Reference water purification system (Millipore, Madrid, Spain) and used for the preparation of all buffers and media.
9
HPLC Analysis of Combination Drugs
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Analysis was carried out using the Agilent 1260 series quaternary gradient HPLC system with an autosampler and photodiode array detector. The METILER TOLEDO analytical balance (MS204S, Switzerland) was used for weighing. The METILER TOLEDO pH meter (S220, Switzerland) was used for pH measurement. The output signal was monitored and processed using Chemstation software (Agilent Technologies, Waldbronn, Germany). The analytical columns screened included Synergi Fusion-RP 80A (150 × 4.6 mm, 4 µm), Luna C18 (250 × 4.6 mm, 5 µm), Luna C8 (250 × 4.6 mm, 5 µm) from Phenomenex (Torrance, CA) and Zorbax SB-C18 (150 × 2.1 mm, 5 µm) from Agilent Technologies (Santa Clara, CA).
BEA was provided by Institute of Microbiology (Chinese Academy of Sciences, Beijing, China). KCZ was purchased from National Institute for Food and Drug Control (Beijing, China). The combination drugs and their corresponding placebo tablets of KCZ and BEA were prepared in our laboratory. High-quality pure water was prepared using the Milli-Q Reference water purification system (Millipore, Molsheim, France). HPLC-grade acetonitrile (ACN) was purchased from Merck (Darmstadt, Germany). HPLC reagent grade potassium dihydrogen phosphate was purchased from Aladdin Industrial Corporation (Shanghai, China). All other reagents were of analytical grade.
BEA was provided by Institute of Microbiology (Chinese Academy of Sciences, Beijing, China). KCZ was purchased from National Institute for Food and Drug Control (Beijing, China). The combination drugs and their corresponding placebo tablets of KCZ and BEA were prepared in our laboratory. High-quality pure water was prepared using the Milli-Q Reference water purification system (Millipore, Molsheim, France). HPLC-grade acetonitrile (ACN) was purchased from Merck (Darmstadt, Germany). HPLC reagent grade potassium dihydrogen phosphate was purchased from Aladdin Industrial Corporation (Shanghai, China). All other reagents were of analytical grade.
10
Trastuzumab Formulation and Preparation
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Trastuzumab (Roche, Basel, Switzerland) was obtained from a local pharmacy. A vial contains a 150 mg molecule as the therapeutic reagent, and it was dissolved in 7.2 mL of pure water to obtain end-solution for injection. L-histidine hydrochloride monohydrate, Lhistidine, α,α-trehalose dihydrate, and polysorbate 20 were supplied from Merck, Germany. Milli-Q ® Reference Water Purification System (Millipore, Burlington, MA, USA) with 18.2 MΩ•cm resistivity was used as a Type 1 water source. Amicon ® Ultra-0.5 mL 50 K centrifugal filter units (PR04190, Millipore, Burlington, MA, USA) were used for buffer exchange. Non-pyrogenic, sterile 0.2 µm PTFE filtration units were obtained from Sarstedt (Numbrecht, Germany). A pH controller was used to adjust the desired pH values.
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