Cd105 phycoerythrin

For immunophenotype characterization, PDLSCs were incubated with antihuman stem cell surface-labeled antibodies including CD105-phycoerythrin, CD45-phycoerythrin, CD146-phycoerythrin, CD14-phycoerythrin, CD29-phycoerythrin, and stro1-phycoerythrin (BD Biosciences, USA). All flow cytometry tests were performed on a FACSAria (BD Bioscience).

After the cell count and viability were measured, isolated cells were washed with DPBS containing 0.2% bovine serum albumin (BSA; Sigma-Aldrich, St. Louis, MO, USA) and 1 mM ethylenediaminetetraacetic acid (EDTA; Gibco). About 1.5 × 10⁵ cells were mixed in each tube with the following antibodies: hCD31–fluorescein isothiocyanate (FITC) (clone: 5.6E; Beckman Coulter, Brea, CA, USA), hCD44–FITC (clone: G44-26; BD Biosciences, Franklin, NJ, USA), hCD45–FITC (clone: J33; Beckman Coulter), hCD73–FITC (clone: AD2; BD Biosciences), hCD81–FITC (clone: JS-81; BD Biosciences), hCD90–allophycocyanin (clone: 5E10; BD Biosciences), and CD105–phycoerythrin (clone: 266; BD Biosciences).
The cells were incubated for 90 min at 4 °C and then washed with DPBS containing 0.2% BSA and 1 mM EDTA. Fluoroprobe-labeled mouse IgG1 antibody (Beckman Coulter) and rat IgG2b antibody (BioLegend, San Diego, CA, USA) were used as negative controls. Stained cells were analyzed using a FACS Verse TM cell sorter (BD Biosciences).