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Sirt1 activator 2

Manufactured by Merck Group
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SIRT1 activator II is a small molecule that has been shown to activate the SIRT1 enzyme in vitro. SIRT1 is a protein deacetylase involved in various cellular processes. This product is intended for research use only.

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Lab products found in correlation

Cerulenin, by Merck Group (2 mentions) Etomoxir, by Merck Group (2 mentions) Allopurinol, by Merck Group (2 mentions) Palmitic, by Cayman Chemical (2 mentions) Oleic acid, by Cayman Chemical (2 mentions) Simvastatin, by Merck Group (2 mentions) Mk 2206, by Cayman Chemical (2 mentions) Af 214 14, by Thermo Fisher Scientific (2 mentions) 25 hydroxycholesterol, by Cayman Chemical (2 mentions) Diphenyleneiodonium chloride dpi, by Merck Group (2 mentions) L ng nitroarginine methyl ester l name, by Cayman Chemical (2 mentions) Ex 527, by Merck Group (2 mentions) Aicar, by Abcam (2 mentions) Hmgcoa, by Merck Group (2 mentions) Cholesterol methyl β cyclodextrin, by Merck Group (2 mentions) D4902, by Merck Group (2 mentions) Compound c, by Merck Group (2 mentions) N acetylcysteine, by Merck Group (2 mentions) Hydrogen peroxide, by Merck Group (2 mentions)

2 protocols using sirt1 activator 2

1

Polarization of Bone Marrow-Derived Macrophages

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
BMMФ were stimulated either with IL-4 (10 ng/ml, AF-214-14, Peprotech), lipopolysaccharide (100 ng/ml, Sigma) or dexamethasone (100nM, D4902, Sigma) to respectively achieve M(IL-4), M(LPS) or M(GC) polarization state. In some experiments, BMMФ were treated with C75 (Sigma), Cerulenin (Sigma), Etomoxir (Sigma), pan-AKT inhibitor MK-2206 (1 mM, Cayman Chemical), 25-hydroxycholesterol (10 μM, Cayman chemical), N-acetyl cysteine (10mM, Sigma), Diphenyleneiodonium chloride (DPI, 5 μM, Sigma), L-NG-Nitroarginine methyl ester (L-NAME, 1mM, Cayman Chemical), Allopurinol (100 μM, Sigma), hydrogen peroxide (Sigma), SIRT1 activator II (Sigma), EX-527 (Sigma), Compound C (Sigma), AICAR (Abcam), HMGCoA (Sigma), water-soluble cholesterol (Cholesterol–methyl-β-cyclodextrin, Sigma) or Simvastatin (Sigma).
For fatty acid treatment, palmitic or oleic acid (Cayman) were solubilized at 100mM in absolute ethanol at 60°C. Fatty acid were conjugated at the desired concentration using a sonicator water bath into the macrophage culture medium to avoid endotoxin contamination from BSA54 (link).
Bidault G., Virtue S., Petkevicius K., Jolin H.E., Dugourd A., Guénantin A.C., Leggat J., Mahler-Araujo B., Lam B.Y., Ma M.K., Dale M., Carobbio S., Kaser A., Fallon P.G., Saez-Rodriguez J., McKenzie A.N, & Vidal-Puig A. (2021). SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation. Nature metabolism, 3(9), 1150-1162.
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2

Polarization of Bone Marrow-Derived Macrophages

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
BMMФ were stimulated either with IL-4 (10 ng/ml, AF-214-14, Peprotech), lipopolysaccharide (100 ng/ml, Sigma) or dexamethasone (100nM, D4902, Sigma) to respectively achieve M(IL-4), M(LPS) or M(GC) polarization state. In some experiments, BMMФ were treated with C75 (Sigma), Cerulenin (Sigma), Etomoxir (Sigma), pan-AKT inhibitor MK-2206 (1 mM, Cayman Chemical), 25-hydroxycholesterol (10 μM, Cayman chemical), N-acetyl cysteine (10mM, Sigma), Diphenyleneiodonium chloride (DPI, 5 μM, Sigma), L-NG-Nitroarginine methyl ester (L-NAME, 1mM, Cayman Chemical), Allopurinol (100 μM, Sigma), hydrogen peroxide (Sigma), SIRT1 activator II (Sigma), EX-527 (Sigma), Compound C (Sigma), AICAR (Abcam), HMGCoA (Sigma), water-soluble cholesterol (Cholesterol–methyl-β-cyclodextrin, Sigma) or Simvastatin (Sigma).
For fatty acid treatment, palmitic or oleic acid (Cayman) were solubilized at 100mM in absolute ethanol at 60°C. Fatty acid were conjugated at the desired concentration using a sonicator water bath into the macrophage culture medium to avoid endotoxin contamination from BSA54 (link).
Bidault G., Virtue S., Petkevicius K., Jolin H.E., Dugourd A., Guénantin A.C., Leggat J., Mahler-Araujo B., Lam B.Y., Ma M.K., Dale M., Carobbio S., Kaser A., Fallon P.G., Saez-Rodriguez J., McKenzie A.N, & Vidal-Puig A. (2021). SREBP1-induced fatty acid synthesis depletes macrophages antioxidant defences to promote their alternative activation. Nature metabolism, 3(9), 1150-1162.
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